Draft Genome Sequence of a non-tuberculous Mycobacterium Strain Isolated from a Clinical Urine Sample.

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Abstract

Here we report the draft sequence of a rapid-growing nontuberculous Mycobacterium isolated from a urine sample at the Scottish Mycobacteria Reference Laboratory (SMRL), Royal Infirmary of Edinburgh, UK. The reported genome has a length of 6,749,454 bp, a GC content of 67.2%, and 6,336 protein-coding sequences. Average nucleotide identity (ANI) analysis identified Mycobacterium vanbaalenii PYR-1 as the closest relative (83.32% ANI), indicating that this isolate likely represents a novel species within the genus. Notably, phenotypic characterisation revealed a distinct antimicrobial resistance (AMR) profile. This assembly provides a valuable resource for studying the evolution of antimicrobial resistance mechanisms in nontuberculous mycobacteria and offers insight into resistance phenotypes observed in clinical isolates.

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