Unveiling the Prevalence of Macrolide Resistant Genes from Streptococcus pneumoniae Isolates in Indonesia
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We determined the prevalence of macrolide resistance genes (mefA and ermB) among isolates of Streptococcus pneumoniae (S. pneumoniae) from Indonesian population. A total of 703 archived S. pneumoniae isolates were screened for the presence of both genes using the polymerase chain reaction. We found total positive isolates for mefA and ermB genes were 10.8% and 4.0% respectively. The major serotype carried mefA gene was 19F (48.1%), followed by serotype 23F (38.8%). The predominant serotype containing ermB genes was 19F (16.9%). The occurrence of the macrolide resistance in S. pneumoniae within Indonesia displays a notable with the most prominent presence observed in serotype 19F.
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Dear Dr Safari, Following confirmation with our editorial office and Chief, I sorry to say the manuscript, in its current state, is no longer under consideration for publication. Several points led to this conclusion. The DNA extraction method is missing, details on why the primer sequences were used are missing (details would be appreciated, reference where possible) and there are no controls mentioned associate with the PCRs. Without a positive control, its unclear if absence of PCR product is due to a failure in PCR/amplification, extraction of DNA or absence of the genes. This positive control (a conserved sequence, e.g. 16s PCR or otherwise) is essential going forward. Best wishes, John.
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