A multi-center study on the percentage of relative abundance, of Klebsiella pneumoniae and Klebsiella oxytoca ,socio-demographic profile and clinical parameters of oral squamous carcinoma tissues of a group of Sri Lankan male patients

  1. Manosha Lakmali Perera
  2. Irosha Perera

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Abstract

Background: Emergence of carbapenemase producing Klebsiella pneumoniae and asymptomatic oral colonization within human populations has become a public health threat Enrichment of oral Klebsiella species in neoplastic remain unclear. Aim: To find out the percentage of relative abundance, of Klebsiella pneumoniae and Klebsiella oxytoca,socio-demographic profile and clinical parameters of oral squamous carcinoma tissues of a group of Sri Lankan male patients in multi-centers. Method: A group of male patients comprising 25 OSCC cases visiting 9 OMF units in 6 Provinces in Sri Lanka represented the vast majority of OSCC patients.  DNA extracted from fresh biopsies was sequenced for the V1 to V3 region with Illumina’s 2 × 300–bp chemistry. High-quality nonchimeric merged reads were classified to the species level with a prioritized BLASTN-based algorithm and identified upto species level.  Downstream compositional analysis was performed with QIIME (Quantitative Insights into Microbial Ecology)  Results: K. pneumoniae was presented an average relative abundance of 1.45%, significantly higher than K. oxytoca, which has a relative abundance of 0.03%. Conclusion: K. pneumoniae over growth was seen compared to K. oxytoca in OSCC cases. This possess an emerging threat of transmitting this pathogen in the community and heath care associated environments as the oral and nasal cavities act as ideal niche for nourishment and flourishment of this opportunistic pathogen, notorious for nosocomial infections and multi drug resistance.

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  1. Access Microbiology

    Dear Dr Perera, Thank you for the submission of your manuscript entitled 'A multi-center study on the percentage of relative abundance, of Klebsiella pneumoniae and Klebsiella oxytoca, socio-demographic profile and clinical parameters of oral squamous carcinoma tissues of a group of Sri Lankan male patients' to Access Microbiology. Our previous communication highlighted that significant proportions of the text have high similarity to previously published articles, specifically relating to 'Persistent enrichment of multidrug‑resistant Klebsiella in oral and nasal communities during long‑term starvation’ (Liu et al. 2024) where lines 209-221 and 229-232 duplicate the abstract wording. Furthermore, a data summary was requested to comply with the Open Data policy of the platform, providing a description of the data generated accompanied by the supporting data that led to the conclusions in the manuscript. The link provided in the data summary section does not provide access to the data used in this manuscript and is instead associated with a review article ‘Emerging role of bacteria in oral carcinogenesis: a review with special reference to perio-pathogenic bacteria’ (Perera et al. 2016). Unfortunately, as these requests were not addressed, we are unable to consider publication of your manuscript in its current format. I would like to thank you for your consideration in publishing your manuscript in Access Microbiology. I believe this retrospective review of data would be of interest to the microbiology community and wish you future success in publication of this work. Yours sincerely, Ray Allan

  2. Version published to 10.1099/acmi.0.001010.v1 on Access Microbiology