Phytochemical screening and in vitro antibacterial evaluation of Plectranthus amboinicus (Lour) spreng against selected four pathogenic bacteria

  1. Abdisha Abrahim Adame
  2. Mulugeta Desta Desta
  3. Zekariya Yusuf Yus
  4. Yusuf Umer Ume
  5. Petros Chavula Chavula

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Abstract

Background Traditional medicine is still utilized around the globe, particularly in developing countries such as Ethiopia, where the majority of people maintain their health through the use of plant medicines. The primary challenges that needed to be addressed were the requirement for scientific backing for adequate documentation and drug development. The current study intended to screen phytochemical and antibacterial activities of Plectranthus amboinicus crude extracts, against selected pathogenic bacteria. Methods The study were designed in Complete Randomized Design based on laboratory bioassay with three replications involves a 2x2x3x4x3 (2 sources i.e. leaf and stem; 2 solvent, i.e. Ethanol, and aqueous water; 3 concentration levels, i.e. 100mg/ml, 120 mg/ml and 150mg/ml; 4 bacterial species; and 3 replications). Qualitative analysis or susceptibility was carried out using standard protocol and phytochemical and antimicrobial activities were determined by disc diffusion and broth dilution method. Result According of study indicated the presence of six phytochemical compounds among the tested compounds, while, Saponin and Terpenoid were absent in ethanol extract and present in water extract. Phlobotanin possess negative at all in both ethanol and water extract. Based on observed susceptibility test the highest inhibition zone 14.6mm was recorded against S. Pyogenes at a concentration of 150 mg/ml, while 9.1 mm inhibition zone was observed against E. coli in concentration of 100 mg/ml. Minimum inhibitory concentration values, in the range 25 mg/mL to 100 mg /ml gram positive and gram negative, minimum bactericidal concentration values ranged 50 mg/ml to 100 mg/ml respectively recorded. Conclusion Plectranthus amboincus plant showed presence of secondary metabolites, possesses bio-active compounds against selected human pathogenic bacterial species, thereby justifying the application of their crude extracts as medicinal plants. Further studies are required against more bacteria and fungus pathogens.

Article activity feed

  1. Access Microbiology

    Dear Msc. Abdisha Abrahim Adame, Your paper has been reviewed by two independent reviewers who raised significant concerns about the results (quantity, quality and presentation) and the conclusions in the paper. Most importantly, the results were found to be still too preliminary to support the conclusions. Therefore, given the extent of the work viewed as necessary to substantiate the conclusions, a decision was made to decline the paper.

  2. Access Microbiology

    Comments to Author

    Dear Author, There are some comments to revise the article, 1. Figure 3 should give information result of bioactive compounds in each tube. 2. Table 1 should be written stem/water and stem/ethanol instead of steam. 3. The conclusion should be more concise and no need to mention table in conclusion. 4. There is repetition "declare that" in declarations. Kind regards,

    Please rate the manuscript for methodological rigour

    Good

    Please rate the quality of the presentation and structure of the manuscript

    Good

    To what extent are the conclusions supported by the data?

    Strongly support

    Do you have any concerns of possible image manipulation, plagiarism or any other unethical practices?

    No

    Is there a potential financial or other conflict of interest between yourself and the author(s)?

    No

    If this manuscript involves human and/or animal work, have the subjects been treated in an ethical manner and the authors complied with the appropriate guidelines?

    Yes

  3. Access Microbiology

    Comments to Author

    The article submitted need to contain more data stating that why the extract is bactericidal , does it contain any antibiotics that can destroy the PG layer of gram positive bacteria. the work seems just a preliminary research.

    Please rate the manuscript for methodological rigour

    Poor

    Please rate the quality of the presentation and structure of the manuscript

    Poor

    To what extent are the conclusions supported by the data?

    Partially support

    Do you have any concerns of possible image manipulation, plagiarism or any other unethical practices?

    No

    Is there a potential financial or other conflict of interest between yourself and the author(s)?

    No

    If this manuscript involves human and/or animal work, have the subjects been treated in an ethical manner and the authors complied with the appropriate guidelines?

    Yes

  4. Version published to 10.1099/acmi.0.000961.v3 on Access Microbiology
  5. Access Microbiology

    Dear Msc. Abdisha Abrahim Adame, After assessing the revised version of the manuscript, I I will have to require a few extra adjustments. First, please remove the pictures showing the work being carried out in the lab. Second, please add legens and describe what is represented in the agar plates and in the tables presented as supplemental material. Third, I suggest you change your statement of intellectual property from "I declare that, this paper was the part of my M.Sc. study thesis. " to "We declare that the text and results presented herein were partially taken from the first author's M.Sc. study thesis". Once you have addressed these issues, you will be able ro resubmit your paper for further review. Best regards, Gustavo

  6. Version published to 10.1099/acmi.0.000961.v2 on Access Microbiology
  7. Access Microbiology

    Dear Msc. Abdisha Abrahim Adame, After assessing your manuscript I noticed that about 40% of the text comes (almost) directly from your Masters thesis. Therefore, I have to ask you to provide a statement making clear that this work reproduces material contained within your Masters thesis. Moreover, I take the opportunity to ask you to make the figure legends more descriptive of what is being shown in the figure. Moreover, tables 2 and 3 must contain units for the antibacterial activity data shown. Finally, figure 4 is very crowded and difficult to understand; you may consider change the figure to make it more accessible to the reader and definitely add a more descriptove legend. Once these issues are addressed, you may resubmit your paper for further consideration. Best regards, Gustavo

  8. Version published to 10.1099/acmi.0.000961.v1 on Access Microbiology