Insights into the genome of Azotobacter sp. strain CWF10, isolated from an agricultural field in Central India, India

  1. Arindam Roy
  2. Anwesha Ghosh
  3. Yash Yash
  4. Prateeksha Mehra
  5. Sumit Roy
  6. Punyasloke Bhadury

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Abstract

Azotobacter sp. strain CWF10, an aerobic gram negative, oval shaped and motile bacterium, was isolated from a lateritic agricultural soil of Madhya Pradesh, India. The draft genome of the isolate is 5.7 Mb in size, consisting of 14 contigs with 65.09 % G+C content. Average nucleotide identity (94.66%) and digital DNA-DNA hybridization (62%) calculation with closest reference strains underpin the bacterium as a potential novel species. The bacterium has a plethora of plant growth promoting genes that point towards potential ability for enhancement of available nitrogen, biosynthesis of folic acid, among others. Siderophores such as vibrioferrin and crochelin A are also present in the genome which are known to regulate iron uptake. Overall, mining of the genome of Azotobacter sp. strain CWF10 has revealed the potential of this strain for application in regenerative agriculture and sustaining soil health.

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  2. Version published to 10.1099/acmi.0.000930.v3 on Access Microbiology
  3. Access Microbiology

    This sentence in the abstract seems to be lacking a word after nitrogen- I would add availability here "The bacterium has a plethora of plant growth promoting genes that point towards potential ability for enhancement of nitrogen, biosynthesis of folic acid, among others." I also would add a sentence to abstract with a summarising statement to enhance the impact of the abstract.

  4. Version published to 10.1099/acmi.0.000930.v2 on Access Microbiology
  5. Access Microbiology

    In addition to the comments raised by the reviewers I would like to ask you to structure your articles in sections: Data summary/ Introduction/ Methods/ Results and Discussion to make it easier to read compared to the current outline.

  6. Access Microbiology

    Comments to Author

    The manuscript presents a detailed overview of the biological nitrogen fixation capabilities of newly identified Azotobacter sp. strain CWF10 and its potential applications in agriculture. However, some points of revision can enhance clarity, coherence, and scientific rigor. * A clear statement mentioning limitations of the study would enhance the overall study * An explanation on the evolving strains of Azotobacter in similar environments and its significance will enhance the finding * Terms such as "novel species" should be defined or clarified to ensure that readers understand the criteria used for this classification. * Ensure that statistical thresholds for species delineation (e.g., ANI

    Please rate the manuscript for methodological rigour

    Good

    Please rate the quality of the presentation and structure of the manuscript

    Good

    To what extent are the conclusions supported by the data?

    Strongly support

    Do you have any concerns of possible image manipulation, plagiarism or any other unethical practices?

    No

    Is there a potential financial or other conflict of interest between yourself and the author(s)?

    No

    If this manuscript involves human and/or animal work, have the subjects been treated in an ethical manner and the authors complied with the appropriate guidelines?

    Yes

  7. Access Microbiology

    Comments to Author

    Through their article entitled "Insights into the genome of Azotobacter sp. strain CWF10, isolated from an agricultural field in Central India, India", Roy et al., announces the genome of a free living N2-fixer with prospective application in plant growth promotion. The article is well drafted and provided details of sequencing protocol performed, QC, and assembly. Few information on annotation might be necessary for annotation segment of the work. The reviewer recommends publication of the article with following modifications. Minor comments: 1. Inclusion of the following annotation data in a tabular format is recommended: total number of coding sequences and predicted genes, Duplicated genes, Protein-encoding genes with functional assignment, Protein-encoding genes without functional assignment, tRNA, rRNA genes, and Under presented genes. 2. Provide detail of model selection for ML tree building. 3. Providing the growth pattern and colony characteristics (or morphological analysis of bacterial cells) of the isolated strain in N2-rich and N2-free medium would have offered a functional data on N2-metabolism.

    Please rate the manuscript for methodological rigour

    Very good

    Please rate the quality of the presentation and structure of the manuscript

    Good

    To what extent are the conclusions supported by the data?

    Strongly support

    Do you have any concerns of possible image manipulation, plagiarism or any other unethical practices?

    No

    Is there a potential financial or other conflict of interest between yourself and the author(s)?

    No

    If this manuscript involves human and/or animal work, have the subjects been treated in an ethical manner and the authors complied with the appropriate guidelines?

    Yes

  8. Version published to 10.1099/acmi.0.000930.v1 on Access Microbiology