Identification of Mammaliicoccus fleurettii as the source of a methicillin-resistant gene in a First Nation reserve lake in Manitoba, Canada
This article has been Reviewed by the following groups
Discuss this preprint
Start a discussion What are Sciety discussions?Listed in
This article is not in any list yet, why not save it to one of your lists.Abstract
Our study aimed to identify the bacterial source of a previously detected mobile antibiotic-resistant gene, mecA , found in a lake that serves as a source to a water treatment plant operated by a First Nation reserve. Three methicillin-resistant presumptive Staphylococcus spp. isolated from the sample using selective media were verified as mecA positive by PCR. MALDI-TOF and whole-genome sequencing of each isolate confirmed that all three were Mammaliicoccus fleurettii . Antibiotic-resistant gene analysis of the assembled genomes predicted mecA with 99.7% sequence identity, and phylogenetic analysis grouped our three mecA genes with the mecA allele from a methicillin-resistant strain of Staphylococcus aureus . Identifying microbial species known to harbour mobile antibiotic-resistant elements can provide greater depth of information about drinking water, an especially essential need in First Nation reserves where water quality too frequently is poor.
