Helicobacter pylori Isolate from Endoscopy Examined Patients in Bahir Dar, North West Ethiopia

  1. Mulat Erkihun
  2. Desalegn Nigatu Chanie
  3. Yesuf Adem Siraj

Reviewed by

Read the full article See related articles

Listed in

This article is not in any list yet, why not save it to one of your lists.
Log in to save this article

Abstract

Background: The Helicobacter pylori infection is highly prevalent throughout the world and causes gastric-associated diseases. Its microbial niche is the stomach, where it causes different abnormalities while being observed. As a result, the current study sought to determine the magnitude and effects of Helicobacter pylori in patients who underwent endoscopic examination. Method: An institution-based cross-sectional study was conducted from January to May 2019 at endoscopy service-providing health institutions. Socio-demographic and clinical data were collected using a pretested, structured questionnaire. Gastric biopsies were collected according to the Sydney protocol during endoscopic procedures and immediately inoculated into brain-heart infusion broth and sub cultured. Then after phenotypic bacterial identification was done. The collected data were, entered, cleaned and analyzed using SPSS version 23. The descriptive statistics was employed for logistic regression model was used to determine crude odds ratio (COR) and adjusted odds ratio (AOR). Then AOR was interpreted with a 95% confidence interval and a significance level less than 0.05. Result:  Among the 135 study participants, 59.3% were males and 40.7% were females, with a mean age of 45 years old. The proportion of Helicobacter pylori isolated was 17.8% (24/135). The majority of isolates (71% of 17/24) were from males, while only 29% of 7/24) were from females. Isolation of Helicobacter pylori was significantly associated with a high salt intake habit [AOR = 3.3, 95% CI = (1.5, 10.8), P = 0.04] and the presence of a duodenal ulcer [AOR = 3.8, 95% CI = (1.2, 11.9), P = 0.02. The highest proportion of Helicobacter pylori had been observed in patients with atrophic pan gastritis. Conclusion: The prevalence of Helicobacter pylori isolated from the study participants was relatively lower. High salt intake and the presence of duodenal ulcers are significantly associated with Helicobacter pylori infection.

Article activity feed

  1. Access Microbiology

    After careful consideration within the editorial board, we regret to inform you that we can no longer consider your manuscript as part of the Access Microbiology platform. The decision to reject your manuscript is based on the sound science requirements of Access Microbiology, as the basis of this study has been published elsewhere (https://onlinelibrary.wiley.com/doi/epdf/10.1155/2023/7326288) and as such, this manuscript does not represent a complete study.

  2. Version published to 10.1099/acmi.0.000751.v2 on Access Microbiology
  3. Access Microbiology

    This manuscript is focusing on an Helicobacter pylori isolate from patients examined with endoscopy. The literature background provided was relevant and adequate, with many recent references of good quality. The methodology was sound and well-described. The Discussion was good but needs a few minor corrections: 1. Highlight the medical importance of your findings 2. Provide more examples of similar Helicobacter pylori cases. Do your results agree with yours? 3. Are your finding only relevant to North West Ethiopia? What about other regions or other African countries? 4. How can your findings help preventing or treating similar H. pylori infections in the future?

  4. Access Microbiology

    Comments to Author

    This is an interesting manuscript on Helicobacter pylori Isolate from Endoscopy Examined Patients. 1. Methodological rigour, reproducibility and availability of underlying data - very good 2. Presentation of results - excellent 3. How the style and organization of the paper communicates and represents key findings - very well 4. Literature analysis or discussion - excellent Minor corrections: - In Abstract, mention values and statistical values, if relevant - Intro: what were the objectives and the hypothesis of this study? - Discussion: are there similar examples from other countries? - Discussion: why are your findings medically-important? how will they help treating similar cases better?

    Please rate the manuscript for methodological rigour

    Very good

    Please rate the quality of the presentation and structure of the manuscript

    Very good

    To what extent are the conclusions supported by the data?

    Strongly support

    Do you have any concerns of possible image manipulation, plagiarism or any other unethical practices?

    No

    Is there a potential financial or other conflict of interest between yourself and the author(s)?

    No

    If this manuscript involves human and/or animal work, have the subjects been treated in an ethical manner and the authors complied with the appropriate guidelines?

    Yes

  5. Version published to 10.1099/acmi.0.000751.v1 on Access Microbiology