Quorum Sensing and Biofilms in Entrobacteriaceae
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Several bacterial species participate in two confirmable behaviours. Irreversible attachment to the surface (biofilm) and to regulate certain phenotypes expression which will synchronize their behaviour (quorum sensing). This sophisticated intercellular communication mechanism called quorum sensing (QS). QS play a major role in the formation of the biofilm & biofilm helps the bacteria in adhesion/ cohesion potentiality, metabolite interchange, cellular connection, defence, nutritious and resistance to medication. Since biofilm is resistant to antibiotics so targeting biofilm is the main therapeutic approach. Biofilm formation is one of the major reason of catheter associated urinary tract infections (CAUTI). E. coli and K. pneumoniae cause Urinary Tract Infection which cost about $1.6 billion in medical expenses every year. Both of them have ability to form biofilms. Exopolysaccharide matrix of biofilm is a double barrier for antibiotics and other antimicrobial substances, therefore makes bacteria more resistant. In many species QS mediated biofilm formation is present, targeting the QS system (anti-QS) are attracting attention in the drug development field. Another treatment strategy is using of bacteriophages. So the aim of our research was to find out bacterial strains able to form biofilms and check the activity of phages able to inhibit formation or eradication of biofilms. We used two species of strains, E. coli and four K. pneumoniae, out of which only E. coli #5 formed biofilm. Bacteriophage specific for E. coli#5 showed that this phage was able to impede the growth of biofilm and also help to eliminate already formed biofilm.
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In this manuscript, Rathore et al. present results about the effect of bacteriophage treatment on the biofilm formation of different enterobacterial strains. I would like to congratulate the authors for contributing with this relevant results, which is a hot topic in the light of the AMR crisis. However, there are major issues that need to be addressed before sending it to peer review. Please find the most important ones highlighted here below: • The use of English needs a big improvement. I would strongly recommend that the authors ask some native English-speaking colleague to proof-read it for them. Alternatively, there is the option of using the services of a language review company, such as Editage (our Editorial Office can provide a discount code for this service if interested). • The manuscript as a whole needs a better …
In this manuscript, Rathore et al. present results about the effect of bacteriophage treatment on the biofilm formation of different enterobacterial strains. I would like to congratulate the authors for contributing with this relevant results, which is a hot topic in the light of the AMR crisis. However, there are major issues that need to be addressed before sending it to peer review. Please find the most important ones highlighted here below: • The use of English needs a big improvement. I would strongly recommend that the authors ask some native English-speaking colleague to proof-read it for them. Alternatively, there is the option of using the services of a language review company, such as Editage (our Editorial Office can provide a discount code for this service if interested). • The manuscript as a whole needs a better structure, and there is a lot of information missing. Examples of this are: 1) there is no information about how the phage (or phages) or the strains were obtained and how the assay was performed needs a lot more clarity; 2) K. pneumoniae strains are mentioned but their results are not shown; 3) there are different references to concepts (for example, fimbriae, quorum sensing (this one even in the title)) that are not further elaborated nor even are related to the results. • The references are shown as a numbered list, but are cited in the text by author surname. Please stick to the Access Micro format (or a single format at least). • The figures cannot be understood by themselves. The Fig. 1 legend is incomplete, and the Fig. 2 legend is absent. There are axis labels missing, the plot formatting complicates the understanding, the Fig. 1 pictures are labeled but the reader cannot know what those labels mean. • Despite the results presented show how phages inhibit biofilm formation in E. coli, the title refers to quorum sensing and Enterobacteria in general, without mentioning phages. The title needs to reflect the content of the manuscript. Please provide a revised version of the manuscript within three months and we will re-assess its progress to peer review.
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