Metagenome-assembled genomes from mineral tundra soils in Rásttigáisá, northern Norway
This article has been Reviewed by the following groups
Discuss this preprint
Start a discussion What are Sciety discussions?Listed in
This article is not in any list yet, why not save it to one of your lists.Abstract
Microbial communities in tundra soils remain largely unknown despite their important roles in the cycling of greenhouse gases. Here, we report 59 non-redundant metagenome-assembled genomes (MAGs) recovered from mineral tundra soils in Rásttigáisá, northern Norway. The MAGs were obtained by clustering contigs according to tetranucleotide frequency and differential coverage and were manually curated to remove contigs with outlying GC content and/or mean coverage. Most MAGs were assigned to the bacterial phyla Candidatus Dormibacterota ( n =12), Verrucomicrobiota ( n =10), and Acidobacteriota ( n =9). All archaeal MAGs ( n =4) belong to the genus Candidatus Nitrosopolaris (phylum Thermoproteota). The 59 Rásttigáisá MAGs expand our knowledge of the diversity and ecological roles of tundra microbiomes.
Article activity feed
-
-
-
The work presented is clear and the arguments well formed. This study would be a valuable contribution to the existing literature. This is a study that would be of interest to the field and community. All comments by the reviewers were satisfactorily addressed.
-
Comments to Author
The authors present metagenome sequencing results from mineral tundra soils from northern Norway. The results include 59 individual genomes assembled from the metagenome data. These genomes have briefly been characterised both in content and taxonomically. Similar to genome announcement papers, the major contribution here is highlighting the availability of the genomic data and contextualising it rather than providing significant in-depth analysis. This said the authors already have provide value from this in another paper highlighted with in. Minor comments are below. No significant changes are recommended beyond expansions and this reviewer congratulates the authors on their work. Very short abstract (
Please rate the manuscript for methodological rigour
Satisfactory
…
Comments to Author
The authors present metagenome sequencing results from mineral tundra soils from northern Norway. The results include 59 individual genomes assembled from the metagenome data. These genomes have briefly been characterised both in content and taxonomically. Similar to genome announcement papers, the major contribution here is highlighting the availability of the genomic data and contextualising it rather than providing significant in-depth analysis. This said the authors already have provide value from this in another paper highlighted with in. Minor comments are below. No significant changes are recommended beyond expansions and this reviewer congratulates the authors on their work. Very short abstract (
Please rate the manuscript for methodological rigour
Satisfactory
Please rate the quality of the presentation and structure of the manuscript
Good
Do you have any concerns of possible image manipulation, plagiarism or any other unethical practices?
No
Is there a potential financial or other conflict of interest between yourself and the author(s)?
No
If this manuscript involves human and/or animal work, have the subjects been treated in an ethical manner and the authors complied with the appropriate guidelines?
Yes
-
Comments to Author
This is an interesting data paper about the sequencing of soil bacteria in Norway. It is well-written, with sound methodology and several good relevant references. Few minor corrections: Abstract: Provide a few sentences in the beginning about soil microbes and your methods. Abstract: At the end, explain why are your findings important for ecology/medicine/industry Future outlook: Expand about similar applications and studies and compare your results with theirs. Future outlook: Highlight the importance of your findings for ecology/medicine/industry Future outlook: Are there any tundra soil studies in other countries?
Please rate the manuscript for methodological rigour
Good
Please rate the quality of the presentation and structure of the manuscript
Good
…
Comments to Author
This is an interesting data paper about the sequencing of soil bacteria in Norway. It is well-written, with sound methodology and several good relevant references. Few minor corrections: Abstract: Provide a few sentences in the beginning about soil microbes and your methods. Abstract: At the end, explain why are your findings important for ecology/medicine/industry Future outlook: Expand about similar applications and studies and compare your results with theirs. Future outlook: Highlight the importance of your findings for ecology/medicine/industry Future outlook: Are there any tundra soil studies in other countries?
Please rate the manuscript for methodological rigour
Good
Please rate the quality of the presentation and structure of the manuscript
Good
Do you have any concerns of possible image manipulation, plagiarism or any other unethical practices?
No
Is there a potential financial or other conflict of interest between yourself and the author(s)?
No
If this manuscript involves human and/or animal work, have the subjects been treated in an ethical manner and the authors complied with the appropriate guidelines?
Yes
-
The work presented is clear and the arguments well formed. This study would be a valuable contribution to the existing literature. This is a study that would be of interest to the field and community. The reviewers have highlighted minor concerns with the work presented. Please ensure that you address their comments.
-