Nicotine inhibits lycopene cyclase in Sphingomonas paucimobilis strain B-54, but not in Sphingomonas echinoides strain B-3126
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Lycopene cyclase-mediated conversion of the non-cyclic lycopene to the bicyclic β-carotene is a crucial step in the carotenogenesis pathway of bacteria, including those of Sphingomonas spp. Nicotine is a potent inhibitor of this enzyme (CrtY), but the molecular mechanism of its action remains to be elucidated. Some Sphingomonas spp. are known to be capable of degrading nicotine, but its effect on carotenoid biosynthesis in these bacteria has not been reported. In this study, the ability of nicotine to inhibit CrtY in Sphingomonas paucimobilis strain B-54 and Sphingomonas echinoides strain B-3126 was investigated, and it was found that cyclic carotenoids were produced in the latter strain even in the presence of the alkaloid. Therefore, it is likely that the CrtY of strain B-3126 escapes the inhibitory effect of nicotine. Further studies using mutation, complementation, and structural characterization are required to understand the differential effects of nicotine in strains B-54 and B-3126.
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The paper is poorly structured and written, which has prevented a proper assessment of the research done. In the current state this manuscript is not suitable to be send out for review for several reasons, please address the following comments in a revised version - there is no subheadings or structure to the article, so it is up to the reader to disentagle what is the introduction, methods, results and the discussion - there is also no explanation/introduction to the motivation/context of the study eg: what is the importance of caretenoids in general and in Sphingomonas, how is nicotine related to caretenoid in nature, why is it important to look at it. What is the aim of this work? What do we gain from this investigation? - why was software used that is outdated (eg MEGA 7 when MEGA 11 is the newest and freely available version) - …
The paper is poorly structured and written, which has prevented a proper assessment of the research done. In the current state this manuscript is not suitable to be send out for review for several reasons, please address the following comments in a revised version - there is no subheadings or structure to the article, so it is up to the reader to disentagle what is the introduction, methods, results and the discussion - there is also no explanation/introduction to the motivation/context of the study eg: what is the importance of caretenoids in general and in Sphingomonas, how is nicotine related to caretenoid in nature, why is it important to look at it. What is the aim of this work? What do we gain from this investigation? - why was software used that is outdated (eg MEGA 7 when MEGA 11 is the newest and freely available version) - it seems there are only UV/VIS spectra of the samples and there seems to be no comparison to a standard, I am not sure that based on the data presented you can identify which caretenoid is produced - in this current format it looks like preliminary data only Unfortunately it is very hard to assess this any further as it is poorly presented.
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