Emendation of cellular fatty acid composition of gliding marine bacteria belonging to the genus Aureispira
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Aureispira marina and Aureispira maritima are gliding marine bacteria with high content of arachidonic acid in cellular lipids isolated from southern coastline of Thailand. In the previous reports (Hosoya, et al., Int. J. Syst. Evol. Microbiol., 56, 2931-2935, 2006, Hosoya et al., Int. J. Syst. Evol. Microbiol., 57, 1948-1951, 2007) only small amounts (less than 5%) of 3-hydroxy fatty acids were detected as cellular hydroxy fatty acids, but recently we found that these bacteria were abundant in 2-hydroxy fatty acids as components of ceramides in their lipids. In the present study cellular fatty acids of these bacteria were reexamined, and the combined ratio of 2-hydroxy fatty acids, composed of iso-17:1 2-OH, iso-17:0 2-OH, and 16:1 2-OH, was shown to be more than 40% of total amount of fatty acids. As the high content of 2-hydroxy fatty acids is a characteristic chemotaxonomic feature of these bacteria, we propose the emendation of cellular fatty acid composition of the above species belonging to the genus Aureispira according to the results in this study.
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The reviewers have highlighted major concerns with the work presented. Please ensure that you address their comments. The reviewers raise concerns regarding the scientific rigour and experimental design of the work.
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Comments to Author
The manuscript by Kawahara et al have looked into the cellular fatty acid composition of gliding marine bacteria represented by 4 strains under the genus Aureispira. I have following observations with the current version of manuscript: (a) The authors stated that these isolates were screened from the coastal water of Thailand and Malaysia. Are these new species or new strain? Please write 2-3 lines in the introduction section so that the readership have a clearer understanding. (b) The salinity of the growth medium has to be stated. What was the optimum growth rate under optimum salinity? (c) Which internal standard was used while undertaking GC-MS approach? (d) How were the samples injected? The authors need to provide more details such as constant flow, oven program, among others. The run time …
Comments to Author
The manuscript by Kawahara et al have looked into the cellular fatty acid composition of gliding marine bacteria represented by 4 strains under the genus Aureispira. I have following observations with the current version of manuscript: (a) The authors stated that these isolates were screened from the coastal water of Thailand and Malaysia. Are these new species or new strain? Please write 2-3 lines in the introduction section so that the readership have a clearer understanding. (b) The salinity of the growth medium has to be stated. What was the optimum growth rate under optimum salinity? (c) Which internal standard was used while undertaking GC-MS approach? (d) How were the samples injected? The authors need to provide more details such as constant flow, oven program, among others. The run time details are absolutely crucial. (e) Did the authors look into cis: trans isomerization? How was R ratio calculated?
Please rate the manuscript for methodological rigour
Good
Please rate the quality of the presentation and structure of the manuscript
Good
To what extent are the conclusions supported by the data?
Strongly support
Do you have any concerns of possible image manipulation, plagiarism or any other unethical practices?
No
Is there a potential financial or other conflict of interest between yourself and the author(s)?
No
If this manuscript involves human and/or animal work, have the subjects been treated in an ethical manner and the authors complied with the appropriate guidelines?
Yes
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Comments to Author
This short manuscript corrects an analytical error in their previous reports of the lipid composition of three (now 4) strains of Aureispira. The manuscript focuses on the lipid with an Rf of 0.8 in the TLC chromatogram. However, the unidentified component with an Rf of 0.5 is not a minor component. My recommendation is that this molecule should be identified and the results should be included in the current manuscript. Usually this reviewer would want separate Results and Discussion sections, but in the present manuscript there are results and supplementary data in the Discussion. For this reason I recommend that the Results and Discussion sections should be combined. Figure legends (Figure 1 and supplementary figures) are at present too short. It is not possible for the reader to understand …
Comments to Author
This short manuscript corrects an analytical error in their previous reports of the lipid composition of three (now 4) strains of Aureispira. The manuscript focuses on the lipid with an Rf of 0.8 in the TLC chromatogram. However, the unidentified component with an Rf of 0.5 is not a minor component. My recommendation is that this molecule should be identified and the results should be included in the current manuscript. Usually this reviewer would want separate Results and Discussion sections, but in the present manuscript there are results and supplementary data in the Discussion. For this reason I recommend that the Results and Discussion sections should be combined. Figure legends (Figure 1 and supplementary figures) are at present too short. It is not possible for the reader to understand their significance without careful reference to the text. The authors should consider labelling more of the components, especially in the supplementary figures. The following editorial changes are also suggested. L - line number L37 contain, not contained L43 replace because of with for L45 but the hydrolysis conditions….. were not strong enough L103 onwards and Table 1. Surely these names of fatty acids are incomplete! L 103 Delete the first sentence and refer to Table 1 at the end of the corrected second sentence. (Table 1)
Please rate the manuscript for methodological rigour
Satisfactory
Please rate the quality of the presentation and structure of the manuscript
Poor
To what extent are the conclusions supported by the data?
Strongly support
Do you have any concerns of possible image manipulation, plagiarism or any other unethical practices?
No
Is there a potential financial or other conflict of interest between yourself and the author(s)?
No
If this manuscript involves human and/or animal work, have the subjects been treated in an ethical manner and the authors complied with the appropriate guidelines?
Yes
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