High levels of eicosanoids and docosanoids in the lungs of intubated COVID‐19 patients

  1. Anne‐Sophie Archambault
  2. Younes Zaid
  3. Volatiana Rakotoarivelo
  4. Caroline Turcotte
  5. Étienne Doré
  6. Isabelle Dubuc
  7. Cyril Martin
  8. Olivier Flamand
  9. Youssef Amar
  10. Amine Cheikh
  11. Hakima Fares
  12. Amine El Hassani
  13. Youssef Tijani
  14. Andréanne Côté
  15. Michel Laviolette
  16. Éric Boilard
  17. Louis Flamand
  18. Nicolas Flamand

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Abstract

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  1. Version published to 10.1096/fj.202100540r
  2. ScreenIT

    SciScore for 10.1101/2020.12.04.20242115: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board StatementIRB: Ethics: This study was approved by the Local Ethics Committee of Cheikh Zaid Hospital (Project: CEFCZ/PR/2020-PR04), Rabat, Morocco and complies with the Declaration of Helsinki and all subjects signed a consent form.
    Consent: Ethics: This study was approved by the Local Ethics Committee of Cheikh Zaid Hospital (Project: CEFCZ/PR/2020-PR04), Rabat, Morocco and complies with the Declaration of Helsinki and all subjects signed a consent form.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.

    Table 2: Resources

    Software and Algorithms
    SentencesResources
    Statistical analysis: Statistical analyses were done using the GraphPad Prism 9 software.
    GraphPad
    suggested: (GraphPad Prism, RRID:SCR_002798)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:
    Nonetheless the expanded definition of a dysregulated lipidome we unravel in SARS-CoV-2 infections requiring mechanical ventilation, our study has limitations. 1) BALs from healthy controls are from younger individuals than those from severe COVID-19 patients. This could have an impact on some mediators, notably PGD2 levels, which are increased in aged mice and worsen SARS-CoV infections (67); 2) The procedure to obtain BAL fluids is slightly different between healthy controls and severe COVID-19 patients. Indeed, while the volume of saline injected to healthy people was 50 ml, that of severe COVID-19 patients was 100 ml. The possible outcome of this discrepancy could be and underestimation of lipid levels in one group vs. the other. While some might argue that the first saline bolus contains more lipids than the following one(s), other might argue that lipid levels are consistent in each bolus. Given that, in our hands, the number of BAL cells is usually greater in the first bolus than in the subsequent ones (when multiple boli are utilized), we are confident that the BAL fluid from the first bolus likely contains more lipids than the second one. This would thus lead to a slight underestimation of lipids in the COVID-19 group. 3) Unfortunately, the leukocyte counts in the BALs of severe COVID-19 patients did not include the number of alveolar macrophages and it is thus impossible to determine a clear differential count of leukocytes in those samples. Furthermore, the eosinop...

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2020.12.04.20242115v1 on medRxiv