DropSynth 2.0: high-fidelity multiplexed gene synthesis in emulsions
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Abstract
Multiplexed assays allow functional testing of large synthetic libraries of genetic elements, but are limited by the designability, length, fidelity and scale of the input DNA. Here, we improve DropSynth, a low-cost, multiplexed method that builds gene libraries by compartmentalizing and assembling microarray-derived oligonucleotides in vortexed emulsions. By optimizing enzyme choice, adding enzymatic error correction and increasing scale, we show that DropSynth can build thousands of gene-length fragments at >20% fidelity.
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Excerpt
Gene Synthesis Costs Reduced to a Drop in the Bucket – DropSynth2.0 Improves Multiplexed Gene Synthesis
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