Rapid detection of neutralizing antibodies to SARS-CoV-2 variants in post-vaccination sera

  1. Kei Miyakawa
  2. Sundararaj Stanleyraj Jeremiah
  3. Hideaki Kato
  4. Yutaro Yamaoka
  5. Hirofumi Go
  6. Satoshi Yajima
  7. Tomoko Shimada
  8. Takahiro Mihara
  9. Atsushi Goto
  10. Takeharu Yamanaka
  11. Akihide Ryo

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Abstract

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  1. Version published to 10.1093/jmcb/mjab050
  2. ScreenIT

    SciScore for 10.1101/2021.05.06.21256788: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    EthicsIRB: Ethics statement: This study was approved by Yokohama City University Certified Institutional Review Board (Reference No. B160800009, B200600115, B210300001), and the protocols used in the study were approved by the ethics committee.
    Consent: Written informed consent was obtained from all the participants.
    Sex as a biological variablenot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Experimental Models: Cell Lines
    SentencesResources
    Production of hiVLP: HEK293 cells (ATCC #CRL-1573), VeroE6/TMPRSS2 cells (JCRB #1819), and VeroE6/TMPRSS2-LgBiT (VTMLG) cells 3 were cultured in DMEM containing 10% FBS.
    HEK293
    suggested: None
    VeroE6/TMPRSS2
    suggested: JCRB Cat# JCRB1819, RRID:CVCL_YQ49)
    Modified hiVNT: VeroE6/TMPRSS2-LgBiT cells seeded in 96-well plates were washed and inoculated with hiVLP stocks (50 µl) containing diluted serum (1:20).
    VeroE6/TMPRSS2-LgBiT
    suggested: None
    Recombinant DNA
    SentencesResources
    hiVLP was produced by transient transfection of HEK293 cells with pHIV-GagPol-HiBiT and pSARS2-Spike-FLAG at a ratio of 1:1.
    pHIV-GagPol-HiBiT
    suggested: None
    pSARS2-Spike-FLAG
    suggested: None
    Software and Algorithms
    SentencesResources
    Statistical analysis: The statistical significance of differences between two groups was evaluated by two-tailed unpaired t-test in the Prism 8 software (GraphPad).
    GraphPad
    suggested: (GraphPad Prism, RRID:SCR_002798)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2021.05.06.21256788v1 on medRxiv