Detection of the SARS-CoV-2 spike protein in saliva with Shrinky-Dink© electrodes
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Abstract
We introduce a simple, scalable, and portable electrochemical aptamer-based system for detection of the SARS-CoV-2 spike protein in saliva with attomolar sensitivity.
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SciScore for 10.1101/2020.11.14.20231811: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:With longer incubation time, however, this limitation was overcome. The outcome was a greater number of moles of aptamers tethered to the surface of SDW electrodes relative to the same geometric area of CD electrodes. The …
SciScore for 10.1101/2020.11.14.20231811: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:With longer incubation time, however, this limitation was overcome. The outcome was a greater number of moles of aptamers tethered to the surface of SDW electrodes relative to the same geometric area of CD electrodes. The increased aptamer density resulted in greater absolute MB peak heights on SDW electrodes. Figure 3B demonstrates the MB peak current densities of CD and SDW electrodes are comparable, with the CD electrodes displaying greater variability as measured by the magnitude of the standard deviation between 3 independent electrodes. To determine whether the introduction of S1 protein to saliva would produce a differentiable signal change relative to untreated saliva, titration curves were built by measuring square wave voltammograms at 10 Hz with SDW electrodes exposed to varying concentrations of S1 protein. The change in signal with increasing concentrations of S1 protein is shown in Figure 3C. A slight shift in the reduction voltage of MB was observed and attributed to binding-induced changes in the local pH at the surface of the electrode. The titration curve displayed in Figure 3D was derived from two electrode sets. One set was incubated in saliva, and the second in saliva spiked with S1 protein. Both sets were incubated in saliva for the same total amount of time. The two separate datasets can be found in Figure S2. As evidenced from Figure 3D, SDW electrodes showed a smaller overall signal change than CD electrodes; we attribute this to the SDW electrode mea...
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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