Reproducibility and sensitivity of 36 methods to quantify the SARS-CoV-2 genetic signal in raw wastewater: findings from an interlaboratory methods evaluation in the U.S.
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Abstract
The reproducibility and sensitivity of 36 methods for quantifying the genetic signal of SARS-CoV-2 in wastewater was evaluated in a nationwide interlaboratory assessment in the U.S.
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SciScore for 10.1101/2020.11.02.20221622: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding The 1-gallon samples were chilled on dry ice to a temperature of approximately 4°C and then blind- spiked with betacoronavirus OC43 to a final concentration of 2.8 x 108 GC/L. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources To prepare the OC43 matrix spike, a concentrated stock of OC43 (Betacoronavirus 1 (ATCC® VR-1558™)) was grown in cell culture using HCT-8 cells (ATCC® CCL-244™), according to ATCC instructions. HCT-8suggested: NoneResults from OddPub: We did not detect open data. We also did …
SciScore for 10.1101/2020.11.02.20221622: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding The 1-gallon samples were chilled on dry ice to a temperature of approximately 4°C and then blind- spiked with betacoronavirus OC43 to a final concentration of 2.8 x 108 GC/L. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources To prepare the OC43 matrix spike, a concentrated stock of OC43 (Betacoronavirus 1 (ATCC® VR-1558™)) was grown in cell culture using HCT-8 cells (ATCC® CCL-244™), according to ATCC instructions. HCT-8suggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a protocol registration statement.
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