Structural basis for the inhibition of SARS-CoV-2 main protease by antineoplastic drug carmofur

SciScore for 10.1101/2020.04.09.033233: (What is this?)

Please note, not all rigor criteria are appropriate for all manuscripts.

Table 1: Rigor

Institutional Review Board Statement	not detected.
Randomization	not detected.
Blinding	not detected.
Power Analysis	not detected.
Sex as a biological variable	not detected.
Cell Line Authentication	Authentication: Vero E6 cells were from ATCC with authentication. Contamination: We confirm that all cells were tested as mycoplasma negative.

Table 2: Resources

Experimental Models: Cell Lines
Sentences	Resources
Vero E6 cells were from ATCC with authentication.	Vero E6 suggested: RRID:CVCL_XD71)
Software and Algorithms
Sentences	Resources
The structure was determined by molecular replacement (MR) with the PHASER22 and Phenix 1.17.123 using the SARS-CoV-2 Mpro (PDB ID: 6LU7) as a search template.	Phenix suggested: (Phenix, …

SciScore for 10.1101/2020.04.09.033233: (What is this?)

Please note, not all rigor criteria are appropriate for all manuscripts.

Table 1: Rigor

Institutional Review Board Statement	not detected.
Randomization	not detected.
Blinding	not detected.
Power Analysis	not detected.
Sex as a biological variable	not detected.
Cell Line Authentication	Authentication: Vero E6 cells were from ATCC with authentication. Contamination: We confirm that all cells were tested as mycoplasma negative.

Table 2: Resources

Experimental Models: Cell Lines
Sentences	Resources
Vero E6 cells were from ATCC with authentication.	Vero E6 suggested: RRID:CVCL_XD71)
Software and Algorithms
Sentences	Resources
The structure was determined by molecular replacement (MR) with the PHASER22 and Phenix 1.17.123 using the SARS-CoV-2 Mpro (PDB ID: 6LU7) as a search template.	Phenix suggested: (Phenix, RRID:SCR_014224)
The model from MR was subsequently subjected to iterative cycles of manual model adjustment with Coot 0.824 and refinement was completed with Phenix REFINE25.	Coot suggested: (Coot, RRID:SCR_014222)

Results from OddPub: Thank you for sharing your data.

Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

Results from TrialIdentifier: No clinical trial numbers were referenced.

Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

Results from JetFighter: We did not find any issues relating to colormaps.

Results from rtransparent:

Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
No protocol registration statement was detected.

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Structural basis for the inhibition of SARS-CoV-2 main protease by antineoplastic drug carmofur

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