Cold sensitivity of the SARS-CoV-2 spike ectodomain
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SciScore for 10.1101/2020.07.12.199588: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Consent: Peripheral blood was collected following informed consent on a Duke University Medical Center approved Institutional Review Board protocol.
IRB: Peripheral blood was collected following informed consent on a Duke University Medical Center approved Institutional Review Board protocol.Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Antibodies were incubated at room temperature for 1 hour, washed and binding detected with goat anti-human-HRP (Jackson ImmunoResearch Laboratories, #109-035-098) and TMB substrate. …SciScore for 10.1101/2020.07.12.199588: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Consent: Peripheral blood was collected following informed consent on a Duke University Medical Center approved Institutional Review Board protocol.
IRB: Peripheral blood was collected following informed consent on a Duke University Medical Center approved Institutional Review Board protocol.Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Antibodies were incubated at room temperature for 1 hour, washed and binding detected with goat anti-human-HRP (Jackson ImmunoResearch Laboratories, #109-035-098) and TMB substrate. anti-human-HRPsuggested: NoneAntibodies captured on a CM5 chip coated with amine-coupled human Anti-Fc (8000RU) were assayed by SARS-CoV-2 spike at 200 nM. Anti-Fcsuggested: NoneExperimental Models: Cell Lines Sentences Resources rS2d-Hexapro was also grown in CHO cells. CHOsuggested: CLS Cat# 603479/p746_CHO, RRID:CVCL_0213)Antibodies were produced in Expi293 cells and purified by Protein A affinity. Expi293suggested: RRID:CVCL_D615)The antibody/virus mixture was then used to inoculate Vero E6 cells (ATCC). Vero E6suggested: RRID:CVCL_XD71)293T/ACE2 cells were kindly provided by Drs. 293T/ACE2suggested: RRID:CVCL_YZ65)Pseudovirions were produced in HEK 293T/17 cells (ATCC cat. no. CRL-11268) by transfection using Fugene 6 (Promega Cat#E2692) and a combination of Spike plasmid, lentiviral backbone plasmid (pCMV ΔR8.2) and firefly Luc reporter gene plasmid (pHR’ CMV Luc)23 in a 1:17:17 ratio. HEK 293T/17suggested: ATCC Cat# CRL-11268, RRID:CVCL_1926)Software and Algorithms Sentences Resources Plasmids were transiently transfected into CHO cells using ExpiFectamine CHO Transfection Kit (ThermoFisher), and protein was purified one the sixth day post-transfection. ThermoFishersuggested: (ThermoFisher; SL 8; Centrifuge, RRID:SCR_020809)The RELION program17 was used for particle picking, 2D and 3D class averaging. RELIONsuggested: (RELION, RRID:SCR_016274)Sensorgram data were analyzed using the BiaEvaluation software (GE Healthcare) BiaEvaluationsuggested: (BIAevaluation Software, RRID:SCR_015936)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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