Fast and highly sensitive full-length single-cell RNA sequencing using FLASH-seq
This article has been Reviewed by the following groups
Listed in
- Evaluated articles (preLights)
Abstract
We present FLASH-seq (FS), a full-length single-cell RNA sequencing (scRNA-seq) method with increased sensitivity and reduced hands-on time compared to Smart-seq3. The entire FS protocol can be performed in ~4.5 hours, is simple to automate and can be easily miniaturized to decrease resource consumption. The FS protocol can also use unique molecular identifiers (UMIs) for molecule counting while displaying reduced strand-invasion artifacts. FS will be especially useful for characterizing gene expression at high resolution across multiple samples.
Article activity feed
-
-
Excerpt
scRNAseq in a FLASH
-
-
