Structural basis for substrate selection by the SARS-CoV-2 replicase
This article has been Reviewed by the following groups
Listed in
- Evaluated articles (ScreenIT)
Abstract
No abstract available
Article activity feed
-
-
SciScore for 10.1101/2022.05.20.492815: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization The experiments were not randomized, and the investigators were not blinded to allocation during experiments and outcome assessment. Blinding The experiments were not randomized, and the investigators were not blinded to allocation during experiments and outcome assessment. Power Analysis No statistical methods were used to predetermine sample size. Table 2: Resources
Recombinant DNA Sentences Resources Briefly, a pQE-30/pcl-ts ind+ plasmid containing a His6-SUMO SARS-CoV-2 nsp12 and untagged nsp7 & 8 (Addgene #160540) was transformed into E. coli BL21 cells (Agilent). pQE-30/pcl-ts ind+suggested: NoneBriefly, the pCDFDuet-1 plasmid … SciScore for 10.1101/2022.05.20.492815: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization The experiments were not randomized, and the investigators were not blinded to allocation during experiments and outcome assessment. Blinding The experiments were not randomized, and the investigators were not blinded to allocation during experiments and outcome assessment. Power Analysis No statistical methods were used to predetermine sample size. Table 2: Resources
Recombinant DNA Sentences Resources Briefly, a pQE-30/pcl-ts ind+ plasmid containing a His6-SUMO SARS-CoV-2 nsp12 and untagged nsp7 & 8 (Addgene #160540) was transformed into E. coli BL21 cells (Agilent). pQE-30/pcl-ts ind+suggested: NoneBriefly, the pCDFDuet-1 plasmid containing His6 SARS-CoV-2 nsp7/8 (Addgene #159092) was transformed into E. coli BL21 (DE3). pCDFDuet-1suggested: RRID:Addgene_15917)Software and Algorithms Sentences Resources Flow-through was collected, concentrated by centrifugal filtration (Amicon), and loaded on a Superdex 200 Hiload 16/600 (Cytiva). Amiconsuggested: NoneThe CTF was estimated for each summed image using the Patch CTF module in cryoSPARC v3.1.0 38. cryoSPARCsuggested: (cryoSPARC, RRID:SCR_016501)Models were inspected and modified in Coot 0.9.5 45 and the refinement process was repeated iteratively. Cootsuggested: (Coot, RRID:SCR_014222)For data processing, the acquired MS spectra were visualized using Thermo Xcalibur Qual Browser (v. 4.2.47). Thermo Xcalibur Qual Browsersuggested: NoneThe quantification and statistical analyses for model refinement and validation were generated using MolProbity 49 and PHENIX 50. MolProbitysuggested: (MolProbity, RRID:SCR_014226)Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
-
