Boceprevir, GC-376, and calpain inhibitors II, XII inhibit SARS-CoV-2 viral replication by targeting the viral main protease
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SciScore for 10.1101/2020.04.20.051581: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Cell lines and viruses: Human rhabdomyosarcoma (RD); A549, MDCK, Caco-2, and Vero cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM), BEAS2B and HCT-8 cells were maintained in RPMI 1640 medium. Caco-2suggested: NoneVerosuggested: NoneBEAS2Bsuggested: NoneCytotoxicity measurement: A549, MDCK, HCT-8, Caco-2, Vero, and BEAS2B cells for cytotoxicity CPE assays were seeded and grown overnight at 37 °C in a 5% CO2 atmosphere … SciScore for 10.1101/2020.04.20.051581: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Cell lines and viruses: Human rhabdomyosarcoma (RD); A549, MDCK, Caco-2, and Vero cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM), BEAS2B and HCT-8 cells were maintained in RPMI 1640 medium. Caco-2suggested: NoneVerosuggested: NoneBEAS2Bsuggested: NoneCytotoxicity measurement: A549, MDCK, HCT-8, Caco-2, Vero, and BEAS2B cells for cytotoxicity CPE assays were seeded and grown overnight at 37 °C in a 5% CO2 atmosphere to ~90% confluence on the next day. A549suggested: NoneMDCKsuggested: NoneHCT-8suggested: NoneBriefly, supernatant virus was serially diluted in log10 increments then plated onto quadruplicate wells of 96-well plates seeded with Vero 76 cells. Vero 76suggested: IZSLER Cat# BS CL 101, RRID:CVCL_0603)Software and Algorithms Sentences Resources The initial velocity was plotted against the FRET concentration with the classic Michaelis-Menten equation in Prism 5 software. Prismsuggested: (PRISM, RRID:SCR_005375)The CC50 values were calculated from best-fit dose-response curves using GraphPad Prism 5 software. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Rigid and restrained refinements were performed using REFMAC and model building with COOT(10, 11). REFMACsuggested: (Refmac, RRID:SCR_014225)Protein structure figures were made using PyMOL (Schrödinger, LLC). PyMOLsuggested: (PyMOL, RRID:SCR_000305)Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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