Small-Molecule Inhibitors of the Coronavirus Spike: ACE2 Protein–Protein Interaction as Blockers of Viral Attachment and Entry for SARS-CoV-2

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1. Version published to 10.1021/acsinfecdis.1c00070

   May 12, 2021
2. 

   ScreenIT

   Oct 26, 2020

   SciScore for 10.1101/2020.10.22.351056: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Briefly, HEK293T cells (ATCC, Manassas, VA, USA; cat. no. CRL-1573) were seeded onto 96-well plates at a density of 50,000 cells per well in 100 μL complete medium (DMEM supplemented with 10% fetal bovine serum).	HEK293T suggested: None
   Software and Algorithms
   Sentences	Resources
   SYPRO Orange (ThermoFisher;	ThermoFisher suggested: (ThermoFisher; SL 8; Centrifuge, RRID:SCR_020809)
   Cell fluorescence was detected using an EVOS FL microscope (Life …

   SciScore for 10.1101/2020.10.22.351056: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Briefly, HEK293T cells (ATCC, Manassas, VA, USA; cat. no. CRL-1573) were seeded onto 96-well plates at a density of 50,000 cells per well in 100 μL complete medium (DMEM supplemented with 10% fetal bovine serum).	HEK293T suggested: None
   Software and Algorithms
   Sentences	Resources
   SYPRO Orange (ThermoFisher;	ThermoFisher suggested: (ThermoFisher; SL 8; Centrifuge, RRID:SCR_020809)
   Cell fluorescence was detected using an EVOS FL microscope (Life Technologies, Carlsbad, CA, USA) and quantified using the Analyze Particles tool after thresholding for the corresponding colors in ImageJ (US National Institutes of Health, Bethesda, MD, USA; (109))	ImageJ suggested: (ImageJ, RRID:SCR_003070)
   As before (56–58), binding data were converted to percent inhibition and fitted with standard log inhibitor vs. normalized response models (63) using nonlinear regression in GraphPad Prism (GraphPad, La Jolla, CA, USA) to establish half-maximal effective or inhibitory concentrations (EC50, IC50).	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798) GraphPad suggested: (GraphPad Prism, RRID:SCR_002798)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Read the original source
3. 

   Version published to 10.1101/2020.10.22.351056v1 on bioRxiv

   Oct 22, 2020