SARS-CoV-2 RNA in Wastewater Settled Solids Is Associated with COVID-19 Cases in a Large Urban Sewershed
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SciScore for 10.1101/2020.09.14.20194472: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization Samples were randomized and blinded to laboratory technicians prior to analysis. RT-QPCR: For method evaluation samples, PMMoV, MHV, BCoV, SARS-CoV-2 N1 and N2 were quantified using two-step RT-QPCR. Blinding Samples were randomized and blinded to laboratory technicians prior to analysis. RT-QPCR: For method evaluation samples, PMMoV, MHV, BCoV, SARS-CoV-2 N1 and N2 were quantified using two-step RT-QPCR. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources QuantaSoft and QuantaSoft Analysis Pro (BioRad) were used to manually threshold and export the data. QuantaSoft…SciScore for 10.1101/2020.09.14.20194472: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization Samples were randomized and blinded to laboratory technicians prior to analysis. RT-QPCR: For method evaluation samples, PMMoV, MHV, BCoV, SARS-CoV-2 N1 and N2 were quantified using two-step RT-QPCR. Blinding Samples were randomized and blinded to laboratory technicians prior to analysis. RT-QPCR: For method evaluation samples, PMMoV, MHV, BCoV, SARS-CoV-2 N1 and N2 were quantified using two-step RT-QPCR. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources QuantaSoft and QuantaSoft Analysis Pro (BioRad) were used to manually threshold and export the data. QuantaSoft Analysis Prosuggested: NoneStatistics: Statistics were computed using Microsoft Excel and Rstudio (version 1.1.463). Microsoft Excelsuggested: (Microsoft Excel, RRID:SCR_016137)Rstudiosuggested: (RStudio, RRID:SCR_000432)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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