Combined Point-of-Care Nucleic Acid and Antibody Testing for SARS-CoV-2 following Emergence of D614G Spike Variant

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1. Version published to 10.1016/j.xcrm.2020.100099

   Sep 1, 2020
2. 

   Version published to 10.1101/2020.06.16.20133157v3 on medRxiv

   Jun 26, 2020
3. 

   Version published to 10.1101/2020.06.16.20133157v2 on medRxiv

   Jun 23, 2020
4. 

   ScreenIT

   Jun 18, 2020

   SciScore for 10.1101/2020.06.16.20133157: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Goat anti-human IgG secondary antibody-Peroxidase (Fc-specific, Sigma) prepared at 1:3,000 in PBST was added and plates were incubated for 1 hour at ambient temperature.	Goat anti-human IgG secondary antibody-Peroxidase (Fc-specific, Sigma) suggested: None anti-human IgG secondary antibody-Peroxidase suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   Confluent 293T cells were transfected with a mixture of 11ul of Fugene HD, 1µg of …

   SciScore for 10.1101/2020.06.16.20133157: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Goat anti-human IgG secondary antibody-Peroxidase (Fc-specific, Sigma) prepared at 1:3,000 in PBST was added and plates were incubated for 1 hour at ambient temperature.	Goat anti-human IgG secondary antibody-Peroxidase (Fc-specific, Sigma) suggested: None anti-human IgG secondary antibody-Peroxidase suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   Confluent 293T cells were transfected with a mixture of 11ul of Fugene HD, 1µg of pCAGGS_SARS-CoV-2_Spike, 1ug of p8.91 HIV-1 gag-pol expression vector(Gupta et al., 2010; Naldini et al., 1996), and 1.5µg of pCSFLW (expressing the firefly luciferase reporter gene with the HIV-1 packaging signal).	293T suggested: None
   Spike protein was expressed and purified from Expi293 cells (Thermo Fisher).	Expi293 suggested: RRID:CVCL_D615)
   Software and Algorithms
   Sentences	Resources
   The EC50 values were calculated with non-linear regression, log (inhibitor) vs. normalized response using GraphPad Prism 8 (GraphPad Software, Inc., San Diego, CA, USA).	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798)
   All data analyses were performed using Prism 8 version 8.4.2 (GraphPad).	Prism suggested: (PRISM, RRID:SCR_005375) GraphPad suggested: (GraphPad Prism, RRID:SCR_002798)
   COVIDIX 2019 SARS-CoV-2 IgG/IgM Test (COVIDIX Healthcare, Cambridge, UK): This colloidal-gold lateral flow immunoassay is designed to detect IgG and IgM to SARS-CoV-2.	COVIDIX Healthcare suggested: None

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: We found the following clinical trial numbers in your paper:
   

   Identifier	Status	Title
   NCT04326387	Recruiting	Evaluation of Novel Diagnostic Tests for COVID-19

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   Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Read the original source
5. 

   Version published to 10.1101/2020.06.16.20133157v1 on medRxiv

   Jun 18, 2020