Metformin suppresses SARS-CoV-2 in cell culture

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  1. SciScore for 10.1101/2021.11.18.469078: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Ethicsnot detected.
    Sex as a biological variablenot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Anti-AMPKα antibody was procured from CST.
    suggested: None
    GAPDH, β-tubulin, and anti-Nucleocapsid antibodies were from ThermoFisher Scientific.
    suggested: None
    suggested: None
    suggested: None
    suggested: None
    Experimental Models: Cell Lines
    Cell culture and reagents: Caco2, Huh7, and Vero cells were grown in DMEM supplemented with FBS, and Pen Strep, at 37°C and 5% CO2.
    suggested: None
    suggested: None
    Cell viability assay: Effect of different doses of metformin on viability of mock and infected Caco2 cells was measured using MTT assay.
    suggested: CLS Cat# 300137/p1665_CaCo-2, RRID:CVCL_0025)
    Software and Algorithms
    Quantification was performed using ImageJ (Schneider et al., 2012).
    suggested: (ImageJ, RRID:SCR_003070)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.