Method versatility in RNA extraction-free PCR detection of SARS-CoV-2 in saliva samples

  1. Orchid M. Allicock
  2. Devyn Yolda-Carr
  3. Rebecca Earnest
  4. Mallery I. Breban
  5. Noel Vega
  6. Isabel M. Ott
  7. Chaney Kalinich
  8. Tara Alpert
  9. Mary E Petrone
  10. Anne L. Wyllie

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Abstract

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  1. Version published to 10.1016/j.pbiomolbio.2023.06.004
  2. ScreenIT

    SciScore for 10.1101/2021.12.27.21268334: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    EthicsIRB: Ethics statement and sample collection: For the spiking experiments, the use of de-identified specimens from healthy or SARS-CoV-2-positive individuals was approved by the Institutional Review Board of the Yale Human Research Protection Program (FWA00002571, Protocol ID. 2000027690) 9.
    Sex as a biological variablenot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.

    Table 2: Resources

    Software and Algorithms
    SentencesResources
    Ethics statement and sample collection: For the spiking experiments, the use of de-identified specimens from healthy or SARS-CoV-2-positive individuals was approved by the Institutional Review Board of the Yale Human Research Protection Program (FWA00002571, Protocol ID. 2000027690) 9.
    Yale Human Research Protection Program
    suggested: None
    All analyses were conducted with GraphPad Prism 9.1.2 (GraphPad Software, Inc., San Diego, CA).
    GraphPad Prism
    suggested: (GraphPad Prism, RRID:SCR_002798)
    GraphPad
    suggested: (GraphPad Prism, RRID:SCR_002798)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2021.12.27.21268334 on medRxiv