Sensitive on-site detection of SARS-CoV-2 by ID NOW COVID-19

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Abstract

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  1. SciScore for 10.1101/2021.04.18.21255688: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Ethicsnot detected.
    Sex as a biological variablenot detected.
    RandomizationFurthermore, 40 swab samples taken from various healthy persons every second day and intermittently positive controls were measured to see whether randomly taken specimens give false-positive results.
    Blindingnot detected.
    Power Analysisnot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Experimental Models: Cell Lines
    SentencesResources
    Finally, cell culture supernatants of a reference strain (SARS-CoV-2/Italy-INMI1, kindly provided by Antonino DiCaro via the GHSAG network), the B.1.1.7 variant (Robert Koch Institute [RKI] isolate), the B.1.351 SA variant (kindly provided by Thorsten Wolff, RKI) and the Brazil TY7-503 variant of the P.1 lineage (hCoV-19/Japan/TY7-503/2021, kindly provided by Takaji Wakita, National Institute of Infectious Diseases, Japan, via the GHSAG network) grown in Vero E6 cells were used to test whether the ID NOW COVID-19 test detects these variants with a similar sensitivity as that for the SARS-CoV-2 variants circulating during the first months of the pandemic.
    Vero E6
    suggested: None
    Recombinant DNA
    SentencesResources
    Finally, cell culture supernatants of a reference strain (SARS-CoV-2/Italy-INMI1, kindly provided by Antonino DiCaro via the GHSAG network), the B.1.1.7 variant (Robert Koch Institute [RKI] isolate), the B.1.351 SA variant (kindly provided by Thorsten Wolff, RKI) and the Brazil TY7-503 variant of the P.1 lineage (hCoV-19/Japan/TY7-503/2021, kindly provided by Takaji Wakita, National Institute of Infectious Diseases, Japan, via the GHSAG network) grown in Vero E6 cells were used to test whether the ID NOW COVID-19 test detects these variants with a similar sensitivity as that for the SARS-CoV-2 variants circulating during the first months of the pandemic.
    hCoV-19/Japan/TY7-503/2021
    suggested: None

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).


    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.


    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.


    Results from JetFighter: We did not find any issues relating to colormaps.


    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.