In vitro evaluation of the effect of mutations in primer binding sites on detection of SARS-CoV-2 by RT-qPCR
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SciScore for 10.1101/2021.06.07.447338: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Experimental Models: Organisms/Strains Sentences Resources Detecting SARS-CoV-2 variant B.1.1.7 with several commercial PCR kits: Extracted RNA of strains mucIMB-1 (wild type SARS-CoV-2) and mucIMB-CB (B.1.1.7 variant) was used with i) the Seegene Allplex SARS-CoV-2/FluA/FluB/RSV assay (Seegene, Seoul, Korea) and run on a Biorad CFX96DX cycler (Hercules, CA, USA), ii) the VitaPCR SARS-CoV-2 assay (Credo Diagnostics, Singapore) and iii) the GeneXpert Xpress SARS-CoV-2 assay (Cepheid, Sunnyvale, CA, USA). mucIMB-1suggested: NoneResults from OddPub: We …
SciScore for 10.1101/2021.06.07.447338: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Experimental Models: Organisms/Strains Sentences Resources Detecting SARS-CoV-2 variant B.1.1.7 with several commercial PCR kits: Extracted RNA of strains mucIMB-1 (wild type SARS-CoV-2) and mucIMB-CB (B.1.1.7 variant) was used with i) the Seegene Allplex SARS-CoV-2/FluA/FluB/RSV assay (Seegene, Seoul, Korea) and run on a Biorad CFX96DX cycler (Hercules, CA, USA), ii) the VitaPCR SARS-CoV-2 assay (Credo Diagnostics, Singapore) and iii) the GeneXpert Xpress SARS-CoV-2 assay (Cepheid, Sunnyvale, CA, USA). mucIMB-1suggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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