Evaluation of spike protein antigens for SARS-CoV-2 serology
This article has been Reviewed by the following groups
Listed in
- Evaluated articles (ScreenIT)
Abstract
No abstract available
Article activity feed
-
-
SciScore for 10.1101/2021.01.27.21250382: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Consent: Informed consent from patients was received as per study protocols approved by the Institute Ethics Committees of the institutes where samples were collected and assays were performed.
IRB: Informed consent from patients was received as per study protocols approved by the Institute Ethics Committees of the institutes where samples were collected and assays were performed.Randomization not detected. Blinding Head-to-head comparison of ST ELISA and EUROIMMUN Anti-SARS-CoV-2 (IgG) ELISA (S1 protein-based serology kit approved by FDA and ICMR, EI 2606-9601 G [22]) was performed in a double-blind format where the experimenters were blind to the RT-PCR and … SciScore for 10.1101/2021.01.27.21250382: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Consent: Informed consent from patients was received as per study protocols approved by the Institute Ethics Committees of the institutes where samples were collected and assays were performed.
IRB: Informed consent from patients was received as per study protocols approved by the Institute Ethics Committees of the institutes where samples were collected and assays were performed.Randomization not detected. Blinding Head-to-head comparison of ST ELISA and EUROIMMUN Anti-SARS-CoV-2 (IgG) ELISA (S1 protein-based serology kit approved by FDA and ICMR, EI 2606-9601 G [22]) was performed in a double-blind format where the experimenters were blind to the RT-PCR and seropositivity results. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources We also tested the control samples for Influenza A/B antibodies (Immunolab Influenza A/B IgG ELISA, ILE-IFA01/ILE-IFB01). ILE-IFA01/ILE-IFB01suggested: NoneThe ST reactivity to SARS specific antibodies was tested with an ELISA titration of the SARS Spike specific antibody CR3022 (Native Antigen MAB12422, Supplementary Figure 1). SARSsuggested: (Imported from the IEDB Cat# CR3022, RRID:AB_2848080)Software and Algorithms Sentences Resources Data analysis: All statistical analyses and visualization were done using custom-written python codes and GraphPad Prism software ( GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
-
