Development and validation of an enzyme-linked immunoassay kit for diagnosis and surveillance of COVID-19
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SciScore for 10.1101/2021.06.23.21259392: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IRB: Sera bank and ethical considerations: The use of sera samples from patients and healthy volunteers was approved by the UFMG’s Ethics Committee and by the National Research Ethics’ Committee (CAAE: 1686320.0.0000.5149). Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Recombinant DNA Sentences Resources Antigen production: The full length coding region of the nucleocapsid (N) gene of SARS-CoV-2 (Genebank accession number: MT126808.1) was codon-optimized, subcloned into pET-24a-(+) expression vector and used to transform E.coli BL21(DE3) strain. pET-24a-suggested: NoneResults from OddPub: Thank you for …
SciScore for 10.1101/2021.06.23.21259392: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IRB: Sera bank and ethical considerations: The use of sera samples from patients and healthy volunteers was approved by the UFMG’s Ethics Committee and by the National Research Ethics’ Committee (CAAE: 1686320.0.0000.5149). Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Recombinant DNA Sentences Resources Antigen production: The full length coding region of the nucleocapsid (N) gene of SARS-CoV-2 (Genebank accession number: MT126808.1) was codon-optimized, subcloned into pET-24a-(+) expression vector and used to transform E.coli BL21(DE3) strain. pET-24a-suggested: NoneResults from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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