SARS-CoV-2 seroprevalence rates of children seeking medical care in Louisiana during the state stay at home order
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SciScore for 10.1101/2020.07.07.20147884: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: The Institutional Review Board of the local academic institution and the children’s hospital from which samples were obtained approved the study protocol (IRB#2020-493). Randomization not detected. Blinding All antibody analyses were performed blind to all medical data including demographic data. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources SARS-CoV-2 IgG ELISA: A serum immunoglobulin G (IgG) ELISA was used to determine presence or absence of SARS-CoV-2 anti-Spike (S) antibodies. anti-Spike (S)suggested: NoneAntibody binding was detected with peroxidase … SciScore for 10.1101/2020.07.07.20147884: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: The Institutional Review Board of the local academic institution and the children’s hospital from which samples were obtained approved the study protocol (IRB#2020-493). Randomization not detected. Blinding All antibody analyses were performed blind to all medical data including demographic data. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources SARS-CoV-2 IgG ELISA: A serum immunoglobulin G (IgG) ELISA was used to determine presence or absence of SARS-CoV-2 anti-Spike (S) antibodies. anti-Spike (S)suggested: NoneAntibody binding was detected with peroxidase conjugated anti-human IgG (Jackson Immunoresearch) and the colorimetric reaction was performed as previously described. anti-human IgGsuggested: NoneExperimental Models: Cell Lines Sentences Resources 16 Antigens were generated by transfection of 293T (ATCC) cells with SARS-CoV-2 S-protein mammalian expression plasmid (BEI Resources). 293Tsuggested: NCBI_Iran Cat# C498, RRID:CVCL_0063)Software and Algorithms Sentences Resources Statistical analysis was performed and graphs created using GraphPad Software v7 and IBM SPSS Statistics (v.25). GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)SPSSsuggested: (SPSS, RRID:SCR_002865)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Despite the strengths of this study, there are limitations. The study population consists of children obtaining medical care during the beginning of the pandemic and is not likely to be representative of the general population. Our data on both race and SES were obtained from electronic medical records. In terms of SES, we utilized estimated average household income from existing data through 2018 and do not suggest that this is a true estimate of the individual SES of the family in which the child lives, but rather is an indicator of the established links between neighborhood factors and health risk. Race was also obtained from electronic medical records and not directly obtained from the participants, as such mixed race categorization is lacking. Our antibody ELISA only captures children who have mounted an antibody response, missing children who have been infected but fail to produce antibodies. It has been reported that some individuals do not produce detectable antibodies after SARS-CoV-2 infection27 and there may be short-term antibody persistence in some individuals. Lastly, the geographical location of the hospital would be most accessible to those residing in Orleans Parish. Children with severe medical emergencies residing in other parishes may have sought care at closer hospitals resulting in less representation of these regions. An enhanced understanding of the general seroprevalence in pediatric populations is critical to implementing public safety protocols rela...
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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