Pushing beyond specifications: Evaluation of linearity and clinical performance of the cobas 6800/8800 SARS-CoV-2 RT-PCR assay for reliable quantification in blood and other materials outside recommendations
This article has been Reviewed by the following groups
Discuss this preprint
Start a discussion What are Sciety discussions?Listed in
- Evaluated articles (ScreenIT)
Abstract
No abstract available
Article activity feed
-
-
SciScore for 10.1101/2020.05.28.20115469: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: The use of anonymized samples was approved by the ethics committee, Freie und Hansestadt Hamburg, PV5626. 3.6 Inter-run variability and comparability of results: Inter-run variability was compared between a total of four different instruments, located at 3 different testing sites. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources For virus quantification, plaque-assays were performed as follows: A tenfold serial dilution series of the virus containing solution was incubated on Vero cells … SciScore for 10.1101/2020.05.28.20115469: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: The use of anonymized samples was approved by the ethics committee, Freie und Hansestadt Hamburg, PV5626. 3.6 Inter-run variability and comparability of results: Inter-run variability was compared between a total of four different instruments, located at 3 different testing sites. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources For virus quantification, plaque-assays were performed as follows: A tenfold serial dilution series of the virus containing solution was incubated on Vero cells seeded in 24-well plates (500 µl per well). Verosuggested: NoneExperimental Models: Organisms/Strains Sentences Resources Target-1 (RdRp gene) and Target-2 (E gene) properties were analysed separately, though the entire assay was deemed positive as long as one Target returned a positive result. Target-1suggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
-
