Pre-clinical evaluation of antiviral activity of nitazoxanide against SARS-CoV-2
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SciScore for 10.1101/2021.12.17.473113: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Consent: Mucilair™ human airway epithelia (HAE), reconstituted from primary cells of bronchial biopsies of a 56-year-old donor Caucasian female with no reported pathologies, was maintained in air liquid interface with specific media (all from Epithelix SARL, Geneva, Switzerland, with informed consent).
IRB: In vivo experiments: Approval and authorization: In vivo experiments were approved by the local ethical committee (C2EA—14) and the French ‘Ministère de l’Enseignement Supérieur, de la Recherche et de l’Innovation’ (APAFIS#23975)
Euthanasia Agents: Euthanasia, which was also realized under general anesthesia, was performed by cervical dislocation.Sex as a biological variable Mucilair™ human … SciScore for 10.1101/2021.12.17.473113: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Consent: Mucilair™ human airway epithelia (HAE), reconstituted from primary cells of bronchial biopsies of a 56-year-old donor Caucasian female with no reported pathologies, was maintained in air liquid interface with specific media (all from Epithelix SARL, Geneva, Switzerland, with informed consent).
IRB: In vivo experiments: Approval and authorization: In vivo experiments were approved by the local ethical committee (C2EA—14) and the French ‘Ministère de l’Enseignement Supérieur, de la Recherche et de l’Innovation’ (APAFIS#23975)
Euthanasia Agents: Euthanasia, which was also realized under general anesthesia, was performed by cervical dislocation.Sex as a biological variable Mucilair™ human airway epithelia (HAE), reconstituted from primary cells of bronchial biopsies of a 56-year-old donor Caucasian female with no reported pathologies, was maintained in air liquid interface with specific media (all from Epithelix SARL, Geneva, Switzerland, with informed consent). Randomization not detected. Blinding Tissue sections of 3.5µm were stained with hematoxylin-eosin (H&E) and blindly analyzed by a certified veterinary pathologist. Power Analysis not detected. Cell Line Authentication Authentication: In vivo experiments: Approval and authorization: In vivo experiments were approved by the local ethical committee (C2EA—14) and the French ‘Ministère de l’Enseignement Supérieur, de la Recherche et de l’Innovation’ (APAFIS#23975) Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Cells and human airway epithelia: VeroE6 cells (ATCC CRL-1586) and Caco-2 cells (ATCC HTB-37) were cultivated under 5% CO2 and at 37.5°C in minimal essential medium (MEM) supplemented with 7.5% heat-inactivated fetal bovine serum (FBS), 1% non-essential amino acids and 1% Penicillin/Streptomycin (all from Life Technologies). VeroE6suggested: NoneCaco-2suggested: NoneSoftware and Algorithms Sentences Resources Graphical representations and statistical analysis: Graphical representations and statistical analyses (two-sided tests when relevant) were performed with Graphpad Prism 7 (Graphpad software). Graphpad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Graphpadsuggested: (GraphPad, RRID:SCR_000306)Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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