SARS-CoV-2 entry sites are present in all structural elements of the human glossopharyngeal and vagal nerves: Clinical implications

  1. Lynn Vitale-Cross
  2. Ildiko Szalayova
  3. Aiden Scoggins
  4. Miklos Palkovits
  5. Eva Mezey

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Abstract

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  1. Version published to 10.1016/j.ebiom.2022.103981
  2. Version published to 10.1101/2021.12.30.474580v3 on bioRxiv
  3. ScreenIT

    SciScore for 10.1101/2021.12.30.474580: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Ethicsnot detected.
    Sex as a biological variablenot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power AnalysisThey were brought to boil at high power (700 W), and then incubated for 5 more minutes at 50% power (350 W).

    Table 2: Resources

    Antibodies
    SentencesResources
    Primary antibodies used: ACE2 (rabbit), Abcam ab15348; NRP1 pre-conjugated to Alexa-488; Neurofilament (chicken), Abcam ab4680; TMPSSR2 (rabbit), Novus Biologicals NBP3-00492; MBP pre-conjugated to Alexa-647; fibronectin (rabbit) gift from Dr. Ken Yamada ([6].
    ACE2
    suggested: None
    Alexa-647; fibronectin
    suggested: None
    Secondary antibodies were purchased from Jackson Laboratories, raised in donkey, anti-rabbit (Cat#711-586-152) or anti-chicken (Cat#ab150170).
    anti-rabbit
    suggested: (Jackson ImmunoResearch Labs Cat# 711-586-152, RRID:AB_2340622)
    anti-chicken
    suggested: (Abcam Cat# ab150170, RRID:AB_2893330)
    Briefly, the slides were incubated overnight at 4 °C with the first primary antibody followed by an anti-IgG for the appropriate species.
    anti-IgG
    suggested: None
    Software and Algorithms
    SentencesResources
    Human primary brain vascular fibroblasts (Cat# H-6076) from Cell Biologics, Inc (Chicago, IL).
    Cell Biologics
    suggested: None

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: Please consider improving the rainbow (“jet”) colormap(s) used on pages 16, 17, 18 and 19. At least one figure is not accessible to readers with colorblindness and/or is not true to the data, i.e. not perceptually uniform.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  4. Version published to 10.1101/2021.12.30.474580v2 on bioRxiv
  5. Version published to 10.1101/2021.12.30.474580v1 on bioRxiv