Developing RT-LAMP assays for rapid diagnosis of SARS-CoV-2 in saliva

Article activity feed

1. Version published to 10.1016/j.ebiom.2021.103736

   Jan 1, 2022
2. 

   ScreenIT

   Apr 28, 2021

   SciScore for 10.1101/2021.04.25.21256085: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	IRB: Assay targets for SARS-CoV-2 diagnosis: This COVID-19 research was approved by the Institutional Review Board at the University of Illinois at Chicago.
   Sex as a biological variable	not detected.
   Randomization	Each 10 µL of RT reaction included 1 µL of 10X RT buffer, 0.4 µL of 25X dNTP (100 mM), 1 µL of 10X random primers, 0.5 µL of reverse transcriptase, 1-5 µL of the RNA sample, and water to final volume of 10 µL.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   HeLa cell RNA: HeLa cells were cultured at 37°C with 5% CO2 in DMEM media supplemented with 10% FBS.	HeLa suggested: None
   …

   SciScore for 10.1101/2021.04.25.21256085: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	IRB: Assay targets for SARS-CoV-2 diagnosis: This COVID-19 research was approved by the Institutional Review Board at the University of Illinois at Chicago.
   Sex as a biological variable	not detected.
   Randomization	Each 10 µL of RT reaction included 1 µL of 10X RT buffer, 0.4 µL of 25X dNTP (100 mM), 1 µL of 10X random primers, 0.5 µL of reverse transcriptase, 1-5 µL of the RNA sample, and water to final volume of 10 µL.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   HeLa cell RNA: HeLa cells were cultured at 37°C with 5% CO2 in DMEM media supplemented with 10% FBS.	HeLa suggested: None
   Software and Algorithms
   Sentences	Resources
   The efficacy of LAMP or RT-LAMP amplification was evaluated by detection of fluorescence signals over a threshold readout (i.e., Time to Threshold, or TT).	LAMP suggested: (LAMP, RRID:SCR_001740)
   The library samples were sequenced on MiniSeq (Illumina).	MiniSeq suggested: None
   In addition, BLAST was performed to identify any sequence match to the NCBI GenBank database.	BLAST suggested: (BLASTX, RRID:SCR_001653)

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   Results from OddPub: Thank you for sharing your data.

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Results from scite Reference Check: We found no unreliable references.

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   Read the original source
3. 

   Version published to 10.1101/2021.04.25.21256085 on medRxiv

   Apr 26, 2021