A CRISPR-Cas12a-based specific enhancer for more sensitive detection of SARS-CoV-2 infection

Article activity feed

1. Version published to 10.1016/j.ebiom.2020.103036

   Nov 1, 2020
2. 

   ScreenIT

   Jun 10, 2020

   SciScore for 10.1101/2020.06.02.20119735: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IRB: All procedures complied with the Measures for the Ethical Review of Biomedical Research Involving Human Subjects issued by the National Health and Family Planning Commission of The People’s Republic of China.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.

   Table 2: Resources

   Software and Algorithms
   Sentences	Resources
   After PCR amplification, samples were sequenced on Illumina Miniseq to produce 2×150 bp paired-end reads.	Miniseq suggested: None
   After adaptor trimming and quality trimming, the clean reads were mapped to the reference genome of SARS-CoV-2 (MN908947.3) using Bowtie2 (22). 4.	Bowtie2 su…

   SciScore for 10.1101/2020.06.02.20119735: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IRB: All procedures complied with the Measures for the Ethical Review of Biomedical Research Involving Human Subjects issued by the National Health and Family Planning Commission of The People’s Republic of China.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.

   Table 2: Resources

   Software and Algorithms
   Sentences	Resources
   After PCR amplification, samples were sequenced on Illumina Miniseq to produce 2×150 bp paired-end reads.	Miniseq suggested: None
   After adaptor trimming and quality trimming, the clean reads were mapped to the reference genome of SARS-CoV-2 (MN908947.3) using Bowtie2 (22). 4.	Bowtie2 suggested: (Bowtie 2, RRID:SCR_016368)

   ---

   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

   ---

   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

   ---

   Results from TrialIdentifier: No clinical trial numbers were referenced.

   ---

   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

   ---

   Results from JetFighter: We did not find any issues relating to colormaps.

   ---

   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • No funding statement was detected.
   • No protocol registration statement was detected.

   ---

   Read the original source
3. 

   Version published to 10.1101/2020.06.02.20119735v1 on medRxiv

   Jun 5, 2020