Clinical, immunological and virological characterization of COVID-19 patients that test re-positive for SARS-CoV-2 by RT-PCR
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SciScore for 10.1101/2020.06.15.20131748: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: Ethics Approval: This study was reviewed and approved by the Medical Ethical Committee of Guangdong Provincial Center for Disease Control and Prevention. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Viral isolation and RT-PCR: Vero E6 cells were inoculated with 100 µl processed patient sample. Vero E6suggested: NoneThereafter, the mixture was added into 96-well Vero-E6 cell culture plate. Vero-E6suggested: NoneSoftware and Algorithms Sentences Resources Sequencing data after mapping to … SciScore for 10.1101/2020.06.15.20131748: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: Ethics Approval: This study was reviewed and approved by the Medical Ethical Committee of Guangdong Provincial Center for Disease Control and Prevention. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Viral isolation and RT-PCR: Vero E6 cells were inoculated with 100 µl processed patient sample. Vero E6suggested: NoneThereafter, the mixture was added into 96-well Vero-E6 cell culture plate. Vero-E6suggested: NoneSoftware and Algorithms Sentences Resources Sequencing data after mapping to SARS-COV-2 reference genome (MN908947.3) have been deposited in the Genome Sequence Archive (11) in BIG Data Center (12), Beijing Institute of Genomics ( Genome Sequence Archivesuggested: NoneStatistical analysis: Statistical analyses were completed using R version 3.5.1 and GraphPad Prism 8.0 (GraphPad Software, Inc. GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Several limitations of our study should also be noted. First, we did not obtain successively collected samples, resulting in the existence of bias toward the summarized duration from the discharge to firstly re-positive result for viral RNA as well as the time of the re-positive RNA to negative. Secondly, we did not obtain the corresponding samples during the acute infection for these re-positive cases. Therefore, some virologic questions remain, including whether there any genetic differences for SARS-CoV-2 viruses sampled in an acute infection phase and a re-positive phase. The significance of SNVs identified in different samples of re-positive cases is limited by the sample size (Figure 3A) and should be further clarified in following studies. Appropriate design intervention strategies on COVID-19 has been largely relied on how well we understand the characteristics of the SARS-CoV-2 infection. Re-positive of viral RNA in some discharged cases could raise a challenge for disease interventions which means a prolonged isolation phase and a more requirement on hospital isolation facilities. Our study result shows a comparable Nab response in re-positive cases comparing to other COVID-19 cases. More importantly, none of infective strains could be successfully isolated and no intact viral genome could be sequenced from all re-positive cases samples highlighting a lower risk for disease transmission from such cases. The additional educations related on SARS-CoV-2 re-positive sho...
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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