Multiple SARS-CoV-2 variants escape neutralization by vaccine-induced humoral immunity

This article has been Reviewed by the following groups

Read the full article See related articles

Listed in

Log in to save this article

Abstract

No abstract available

Article activity feed

  1. Biliang Zhang, Caroline Zhang

    Review 1: "Circulating SARS-CoV-2 variants escape neutralization by vaccine-induced humoral immunity"

    Reviewers: Biliang Zhang (Guangzhou Institutes of Biomedicine and Health) and Caroline Zhang (Brandeis University) | 📗📗📗📗◻️

  2. Biliang Zhang, Caroline Zhang

    Review of "Circulating SARS-CoV-2 variants escape neutralization by vaccine-induced humoral immunity"

    Reviewers: Biliang Zhang (Guangzhou Institutes of Biomedicine and Health) and Caroline Zhang (Brandeis University) | 📗📗📗📗◻️

  3. SciScore for 10.1101/2021.02.14.21251704: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board Statementnot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    Cells were then washed with 1 mL PBS+, spun at 900 x g, and stained with 2 µg/mL of anti-human IgG-AF647 polyclonal antibody (Invitrogen) for 30 minutes at room temperature.
    anti-human IgG-AF647
    suggested: (SouthernBiotech Cat# 2040-31, RRID:AB_2795651)
    SARS-CoV-2 receptor binding domain and spike IgG, IgM, and IgA ELISA: Quantitative detection of total antibodies to SARS-CoV-2 receptor binding domain (RBD) was performed as previously described (Garcia-Beltran et al. 2021).
    SARS-CoV-2 receptor binding domain and spike IgG
    suggested: None
    Briefly, we used an indirect ELISA with a standard consisting of anti-SARS-CoV and -CoV-2 monoclonal antibody (CR3022) (IgG1 isotype).
    anti-SARS-CoV
    suggested: None
    -CoV-2
    suggested: (Imported from the IEDB Cat# CR3022, RRID:AB_2848080)
    CR3022
    suggested: None
    (IgG1
    suggested: None
    Total antibodies were detected with anti-human IgG/A/M(H+L)-HRP (Bethyl) diluted 1:25,000 for a 30 min incubation at room temperature.
    anti-human IgG/A/M(H+L)-HRP
    suggested: None
    Anti-RBD antibody levels were calculated by interpolating onto the standard curve and correcting for sample dilution; one unit per mL (U/mL) was defined as the equivalent reactivity seen by 1 µg/mL of CR3022.
    Anti-RBD
    suggested: None
    Experimental Models: Cell Lines
    SentencesResources
    Following collection and filtering, production was quantified by titering via flow cytometry on 293T-ACE2 cells.
    293T-ACE2
    suggested: RRID:CVCL_YZ65)
    Spike Expression: To compare the relative surface expression of pseudovirus spike variant proteins, we plated 400,000 293T cells per well of a 12-well plate.
    293T
    suggested: None
    Software and Algorithms
    SentencesResources
    Data was analyzed using Graphpad Prism and NT50 values were calculated by taking the inverse of the 50% inhibitory concentration value for all samples with a neutralization value of 80% or higher at the highest concentration of serum.
    Graphpad Prism
    suggested: (GraphPad Prism, RRID:SCR_002798)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).


    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.


    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.


    Results from JetFighter: We did not find any issues relating to colormaps.


    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.