Multiple SARS-CoV-2 variants escape neutralization by vaccine-induced humoral immunity
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Biliang Zhang, Caroline Zhang
Review 1: "Circulating SARS-CoV-2 variants escape neutralization by vaccine-induced humoral immunity"
Reviewers: Biliang Zhang (Guangzhou Institutes of Biomedicine and Health) and Caroline Zhang (Brandeis University) | 📗📗📗📗◻️
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Biliang Zhang, Caroline Zhang
Review of "Circulating SARS-CoV-2 variants escape neutralization by vaccine-induced humoral immunity"
Reviewers: Biliang Zhang (Guangzhou Institutes of Biomedicine and Health) and Caroline Zhang (Brandeis University) | 📗📗📗📗◻️
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SciScore for 10.1101/2021.02.14.21251704: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Cells were then washed with 1 mL PBS+, spun at 900 x g, and stained with 2 µg/mL of anti-human IgG-AF647 polyclonal antibody (Invitrogen) for 30 minutes at room temperature. anti-human IgG-AF647suggested: (SouthernBiotech Cat# 2040-31, RRID:AB_2795651)SARS-CoV-2 receptor binding domain and spike IgG, IgM, and IgA ELISA: Quantitative detection of total antibodies to SARS-CoV-2 receptor binding domain (RBD) was performed as previously described … SciScore for 10.1101/2021.02.14.21251704: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Cells were then washed with 1 mL PBS+, spun at 900 x g, and stained with 2 µg/mL of anti-human IgG-AF647 polyclonal antibody (Invitrogen) for 30 minutes at room temperature. anti-human IgG-AF647suggested: (SouthernBiotech Cat# 2040-31, RRID:AB_2795651)SARS-CoV-2 receptor binding domain and spike IgG, IgM, and IgA ELISA: Quantitative detection of total antibodies to SARS-CoV-2 receptor binding domain (RBD) was performed as previously described (Garcia-Beltran et al. 2021). SARS-CoV-2 receptor binding domain and spike IgGsuggested: NoneBriefly, we used an indirect ELISA with a standard consisting of anti-SARS-CoV and -CoV-2 monoclonal antibody (CR3022) (IgG1 isotype). anti-SARS-CoVsuggested: None-CoV-2suggested: (Imported from the IEDB Cat# CR3022, RRID:AB_2848080)CR3022suggested: None(IgG1suggested: NoneTotal antibodies were detected with anti-human IgG/A/M(H+L)-HRP (Bethyl) diluted 1:25,000 for a 30 min incubation at room temperature. anti-human IgG/A/M(H+L)-HRPsuggested: NoneAnti-RBD antibody levels were calculated by interpolating onto the standard curve and correcting for sample dilution; one unit per mL (U/mL) was defined as the equivalent reactivity seen by 1 µg/mL of CR3022. Anti-RBDsuggested: NoneExperimental Models: Cell Lines Sentences Resources Following collection and filtering, production was quantified by titering via flow cytometry on 293T-ACE2 cells. 293T-ACE2suggested: RRID:CVCL_YZ65)Spike Expression: To compare the relative surface expression of pseudovirus spike variant proteins, we plated 400,000 293T cells per well of a 12-well plate. 293Tsuggested: NoneSoftware and Algorithms Sentences Resources Data was analyzed using Graphpad Prism and NT50 values were calculated by taking the inverse of the 50% inhibitory concentration value for all samples with a neutralization value of 80% or higher at the highest concentration of serum. Graphpad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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