Standardization of a flow cytometry SARS-CoV-2 serologic test
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SciScore for 10.1101/2021.01.29.21250770: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Consent: All donors gave consent to participate in this research project which was approved by the Héma-Québec Research Ethic Committee.
IRB: All donors gave consent to participate in this research project which was approved by the Héma-Québec Research Ethic Committee.Randomization Convalescents plasmas were randomly chosen from adult participants in the CONCOR-1 study (#NCT04348656) having received an official diagnosis of COVID-19 by the Québec Provincial Health Authority confirmed by polymerase chain reaction (PCR). Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
… SciScore for 10.1101/2021.01.29.21250770: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Consent: All donors gave consent to participate in this research project which was approved by the Héma-Québec Research Ethic Committee.
IRB: All donors gave consent to participate in this research project which was approved by the Héma-Québec Research Ethic Committee.Randomization Convalescents plasmas were randomly chosen from adult participants in the CONCOR-1 study (#NCT04348656) having received an official diagnosis of COVID-19 by the Québec Provincial Health Authority confirmed by polymerase chain reaction (PCR). Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources After washing, AlexaFluor-647-conjugated goat anti-human IgG+IgA+IgM Abs (Jackson Immunoresearch 109-605-064) diluted 1:400 (final concentration of 3.75 µg/ml) was used as detection antibody for 20 minutes at room temperature. anti-human IgG+IgA+IgM Abssuggested: Noneanti-human IgG+IgA+IgMsuggested: (Jackson ImmunoResearch Labs Cat# 109-605-064, RRID:AB_2337886)Experimental Models: Cell Lines Sentences Resources Briefly, a mix of 293T-SARS-CoV-2 Spike stable cell line and 293T control cells were stained with an anti-RBD CR3022 monoclonal Ab (50 ng/ml) (graciously provided by A. Finzi) as control or plasma (1:400 dilution) for 20 minutes at room temperature. 293Tsuggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: We found the following clinical trial numbers in your paper:
Identifier Status Title NCT04348656 Recruiting CONvalescent Plasma for Hospitalized Adults With COVID-19 Re… Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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