Assessment of Maternal and Neonatal Cord Blood SARS-CoV-2 Antibodies and Placental Transfer Ratios

  1. Dustin D. Flannery
  2. Sigrid Gouma
  3. Miren B. Dhudasia
  4. Sagori Mukhopadhyay
  5. Madeline R. Pfeifer
  6. Emily C. Woodford
  7. Jourdan E. Triebwasser
  8. Jeffrey S. Gerber
  9. Jeffrey S. Morris
  10. Madison E. Weirick
  11. Christopher M. McAllister
  12. Marcus J. Bolton
  13. Claudia P. Arevalo
  14. Elizabeth M. Anderson
  15. Eileen C. Goodwin
  16. Scott E. Hensley
  17. Karen M. Puopolo

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Abstract

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  1. Version published to 10.1001/jamapediatrics.2021.0038
  2. ScreenIT

    SciScore for 10.1101/2020.10.07.20207480: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board StatementIRB: 4 Ethics approval: The Institutional Review Board of the University of Pennsylvania approved this study with waiver of informed consent.
    Consent: 4 Ethics approval: The Institutional Review Board of the University of Pennsylvania approved this study with waiver of informed consent.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablePregnant women at Pennsylvania Hospital have blood drawn for rapid plasma reagin at the time of delivery as routine syphilis screening and newborns routinely have cord blood stored if blood type or Coombs testing is clinically needed; residual maternal and cord blood serum from this testing was obtained when it would otherwise be discarded.

    Table 2: Resources

    Antibodies
    SentencesResources
    After 2 hours incubation at room temperature using a plate mixer, plates were washed again 3 times with PBS-T and 50 µL of 1:5000 diluted horseradish peroxidase labeled goat anti-human IgG (Jackson ImmunoResearch Laboratories) or 1:1000 diluted goat anti-human IgM-horseradish peroxidase (SouthernBiotech) secondary antibodies were added.
    1:1000 diluted goat anti-human IgM-horseradish peroxidase (SouthernBiotech) secondary antibodies
    suggested: None
    anti-human IgM-horseradish peroxidase
    suggested: None
    Monoclonal antibody CR3022 was included on each plate to adjust for plate-to-plate variation and antibody concentrations were expressed in arbitrary units relative to CR3022.
    CR3022
    suggested: None
    Software and Algorithms
    SentencesResources
    Statistical analyses were performed using Stata version 16 (StataCorp, College Station, TX) and Prism version 8 (GraphPad Software).
    StataCorp
    suggested: (Stata, RRID:SCR_012763)
    Prism
    suggested: (PRISM, RRID:SCR_005375)
    GraphPad
    suggested: (GraphPad Prism, RRID:SCR_002798)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2020.10.07.20207480 on medRxiv