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- Ivaine Tais Sauthier Sartor
- Fernanda Hammes Varela
- Mariana Rost Meireles
- Luciane Beatriz Kern
- Thaís Raupp Azevedo
- Gabriela Luchiari Tumioto Giannini
- Mariana Soares da Silva
- Meriane Demoliner
- Juliana Schons Gularte
- Paula Rodrigues de Almeida
- Juliane Deise Fleck
- Gabriela Oliveira Zavaglia
- Ingrid Rodrigues Fernandes
- Caroline Nespolo de David
- Amanda Paz Santos
- Walquiria Aparecida Ferreira de Almeida
- Victor Bertollo Gomes Porto
- Marcelo Comerlato Scotta
- Gustavo Fioravanti Vieira
- Fernando R. Spilki
- Renato T. Stein
- Márcia Polese-Bonatto
- COVIDa study group
The emergence of SARS-CoV-2 variants is a current public health concern possibly impacting COVID-19 disease diagnosis, transmission patterns and vaccine effectiveness.
To describe the SARS-CoV-2 lineages circulating early pandemic among samples with S gene dropout and characterize a novel mutation in receptor-binding domain (RBD) of viral spike protein.
Adults and children older than 2 months with signs and symptoms of COVID-19 were prospectively enrolled from May to October 2020 in Porto Alegre, Brazil. All participants performed RT-PCR assays for diagnosing SARS-CoV-2, samples with S gene dropout and Ct < 30 (cycle threshold) were submitted to whole genome sequencing (WGS), and homology modeling and physicochemical properties analysis were performed.
484/1,557 participants tested positive for SARS-CoV-2. The S gene dropout was detected in 7.4% (36/484) as early as May, and a peak was observed in early August. WGS was performed in 8 samples. The B.1.1.28, B.1.91 and B.1.1.33 lineages were circulating in early pandemic. The RBD novel mutation (Y380Q) was found in one sample occurring simultaneously with C379W and V395A, and the B.1.91 lineage in the spike protein.
Mutations in the SARS-CoV-2 spike region were detected early in the COVID-19 pandemic in Southern Brazil, regarding the B.1.1.28, B.1.91 and B.1.1.33 lineages identified. The novel mutation (Y380Q) with C379W, modifies important RBD properties, which may interfere with the binding of neutralizing antibodies (CR3022, EY6A, H014, S304).
Characterization of novel mutation (Y380Q) in RBD of SARS-CoV-2 spike protein
The Y380Q and C379W modify important properties in the SARS-CoV-2 RBD region
The RBD mutations may interfere with the binding of neutralizing antibodies
The B.1.1.28, B.1.91 and B.1.1.33 lineages were circulating in early pandemic