Comparative Performance of Four Nucleic Acid Amplification Tests for SARS-CoV-2 Virus
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SciScore for 10.1101/2020.03.26.010975: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: The study was approved by the Ethics Committees from the Guangdong Provincial Hospital of Chinese Medicine (ZE2020-027-01). Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication Contamination: Analytical specificity: Eighteen pseudovirus samples of analytical specificity reference materials from the performance verification reference material kit were used to determine the analytical specificity of four NAATs, including human coronavirus HCoV-OC43, HCoV-HKU1 RNA, HCoV-229E RNA, HCoV-NL63 RNA, SARS RNA, Middle East respiratory syndrome (MERS) RNA, influenza A HIN1 virus, … SciScore for 10.1101/2020.03.26.010975: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: The study was approved by the Ethics Committees from the Guangdong Provincial Hospital of Chinese Medicine (ZE2020-027-01). Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication Contamination: Analytical specificity: Eighteen pseudovirus samples of analytical specificity reference materials from the performance verification reference material kit were used to determine the analytical specificity of four NAATs, including human coronavirus HCoV-OC43, HCoV-HKU1 RNA, HCoV-229E RNA, HCoV-NL63 RNA, SARS RNA, Middle East respiratory syndrome (MERS) RNA, influenza A HIN1 virus, influenza B INFB virus, respiratory syncytial virus type A and type B, human parainfluenza virus, adenovirus, enterovirus, mycoplasma pneumoniae, Epstein–Barr (EB) virus, human cytomegalovirus, Mycobacterium tuberculosis, and two samples with human genome DNA. Table 2: Resources
Software and Algorithms Sentences Resources Statistical analysis: The significance of the difference in sensitivity between the NAATs was assessed by using Fisher’s exact test in GraphPad Prism (5.0). GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)The 95% confidence intervals (CIs) for specificity and sensitivity were calculated by using the Wilson–Score method in GraphPad Prism. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- No conflict of interest statement was detected. If there are no conflicts, we encourage authors to explicit state so.
- No funding statement was detected.
- No protocol registration statement was detected.
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