Does the human placenta express the canonical cell entry mediators for SARS-CoV-2?

  1. Roger Pique-Regi
  2. Roberto Romero
  3. Adi L Tarca
  4. Francesca Luca
  5. Yi Xu
  6. Adnan Alazizi
  7. Yaozhu Leng
  8. Chaur-Dong Hsu
  9. Nardhy Gomez-Lopez

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Abstract

The pandemic of coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected more than 10 million people, including pregnant women. To date, no consistent evidence for the vertical transmission of SARS-CoV-2 exists. The novel coronavirus canonically utilizes the angiotensin-converting enzyme 2 (ACE2) receptor and the serine protease TMPRSS2 for cell entry. Herein, building upon our previous single-cell study (Pique-Regi et al., 2019), another study, and new single-cell/nuclei RNA-sequencing data, we investigated the expression of ACE2 and TMPRSS2 throughout pregnancy in the placenta as well as in third-trimester chorioamniotic membranes. We report that co-transcription of ACE2 and TMPRSS2 is negligible in the placenta, thus not a likely path of vertical transmission for SARS-CoV-2. By contrast, receptors for Zika virus and cytomegalovirus, which cause congenital infections, are highly expressed by placental cell types. These data show that the placenta minimally expresses the canonical cell-entry mediators for SARS-CoV-2.

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  1. Version published to 10.7554/elife.58716 on eLife
  2. ScreenIT

    SciScore for 10.1101/2020.05.18.101485: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board Statementnot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.

    Table 2: Resources

    Software and Algorithms
    SentencesResources
    Data availability: Placenta and decidua scRNA-seq data from first-trimester samples were downloaded through ArrayExpress (E-MTAB-6701).
    ArrayExpress
    suggested: (ArrayExpress, RRID:SCR_002964)
    Visualization of viral receptor gene expression was performed using the ggplot2 [104] package in R with gene expression values scaled to transcripts per million (TPM).
    ggplot2
    suggested: (ggplot2, RRID:SCR_014601)
    The mas5calls function from the affy package in Bioconductor was used to determine presence above background of each probeset corresponding to a given gene [105].
    affy
    suggested: (affy, RRID:SCR_012835)
    Bioconductor
    suggested: (Bioconductor, RRID:SCR_006442)

    Results from OddPub: Thank you for sharing your code and data.

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • No conflict of interest statement was detected. If there are no conflicts, we encourage authors to explicit state so.
    • No funding statement was detected.
    • No protocol registration statement was detected.

    About SciScore

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  3. Version published to 10.1101/2020.05.18.101485v1 on bioRxiv