Development of a Saliva-Optimized RT-LAMP Assay for SARS-CoV-2
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SciScore for 10.1101/2020.12.26.20248880: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Importantly however, the number of channels is constrained by mechanical considerations in multichannel pipettes, whereas the MS simplicity enables the number of simultaneous “channels” to scale well beyond traditional …
SciScore for 10.1101/2020.12.26.20248880: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Importantly however, the number of channels is constrained by mechanical considerations in multichannel pipettes, whereas the MS simplicity enables the number of simultaneous “channels” to scale well beyond traditional limitations. Indeed, we developed 4-channel, 8-channel, and a 24-channel versions for the purpose of large-scale multiplex purification reactions, as well as a custom rack designed for a 24-channel MS to elute directly into a 96 or 384-well plate (Figure 9B and 9C). Second, this superior scaling potential also reduces the additional processing time required by more samples. The addition of every 8 samples increases the required processing time with an 8-channel multipipette, but a 24-channel MS increases processing time only beyond 24 samples. The processing time is further reduced because 2-3 pipetting steps are replaced with a single dipping step. Third, the cost of a MS is dramatically less than a multichannel pipette. A 24-channel magnetic stick costs less than $5.00 to produce, and each tip is less than $0.05, about $6.00 in total. An 8-channel multipipette in contrast costs well over a thousand dollars. Our purification optimization results also suggest several unexplored directions for future saliva-based nucleic acid diagnostics. The optimal NaCl concentrations were well below the theoretical limit required for purifying nucleic acids, suggesting that uncharacterized minerals or salts present in saliva are aiding nucleic acid binding to carboxylated bea...
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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