Phosphorylation of Z granule components regulates germ granule organization and RNAi inheritance
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Small RNAs represent important carriers of epigenetic information in nematodes, and factors involved in their biogenesis reside within perinuclear germ granules. Among these condensates, Z granules are essential for the inheritance of RNA interference (RNAi). The mechanisms by which Z granules form and promote this process remain unclear. We show that Casein kinase I alpha (KIN-19) is present in Z granules and associated with DEPS-1 and HERD-1 phosphorylation. In the absence of DEPS-1 and HERD-1 phosphorylation, animals have enlarged Z granules and enhanced RNAi inheritance. We propose that DEPS-1 acts as a phosphorylation-regulated scaffold that bridges ZNFX-1 and HERD-1 proteins. Our study demonstrates that Casein kinase I alpha-mediated phosphorylation is an important factor in germ granule modulation and RNAi inheritance, and additionally provides a novel molecular scaffold for understanding germ granule complexity in C. elegans .
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A preprint using: L4440 (RRID:Addgene_1654) from @addgene.bsky.social was published. SciScore made a table with this resource, see “Automated Services” module (download as csv, xml or #jats) #OpenScience #methodsmatter
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Phosphorylation of Z granule components regulates germ granule organization and RNAi inheritance https://www.biorxiv.org/content/10.64898/2026.10.06.756944v1
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Phosphorylation of Z granule components regulates germ granule organization and RNAi inheritance https://www.biorxiv.org/content/10.64898/2026.10.06.756944v1
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