μMap-uAA: Photocatalytic proximity labeling targeted with single-residue precision
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Mapping protein microenvironments with residue-level precision in living cells remains challenging. We report μMap-uAA, a genetically encoded proximity labeling platform that uses unnatural amino acid incorporation to install a tetrazine-quenched iridium photocatalyst at user-defined protein sites through click chemistry. Photocatalysis is activated by covalent attachment to the incorporated uAA, enabling localized catalytic labeling of proximal biomolecules. Applied to membrane receptors, μMap-uAA captures domain- and time-dependent GPCR interaction networks with residue-level spatial precision.
