Distinct Evolutionary Trajectories in Early Lung Adenocarcinoma: Age-Related Pathway with Epidermal Growth Factor Receptor–Associated Genome Doubling and Smoking-Driven Pathway
Discuss this preprint
Start a discussion What are Sciety discussions?Listed in
This article is not in any list yet, why not save it to one of your lists.Abstract
Lung adenocarcinoma a progress from preinvasive lesions to invasive cancer; however, early evolutionary events in adenocarcinoma in situ (AIS) and minimally invasive adenocarcinoma (MIA) remain poorly defined, particularly in East Asian populations enriched for EGFR mutations. We performed whole-exome sequencing on 67 Japanese patients (38 AIS, 29 MIA), including multiregion sampling in 13 cases to identify EGFR as predominant driver (61.2%), followed by RBM10 (19.4%) and TP53 (9.0%). Two evolutionary trajectories emerged: age-related and smoking-driven pathways. In the former, EGFR mutant tumors frequently exhibited early whole genome doubling (WGD) (24.4%) with clock like signature. The smoking driven pathway, typically involving KRAS mutations, displayed a tobacco associated signature. Multiregion sequencing revealed that driver mutations (EGFR , KRAS , and MET ) were shared trunk events across in situ and invasive regions, while secondary alterations arose subclonally. This study defines the genomic evolution of early lung adenocarcinoma in Japanese patients, identifying two evolutionary trajectories: an age-related pathway with EGFR- linked genome doubling and a smoking-driven pathway involving KRAS mutations. These findings elucidate mechanisms underlying progression from preinvasive lesions to invasive cancer in Asian populations.
