Generalized Chargaff symmetry in codon usage across the tree of life

Codon usage bias is a central record of mutation, selection, drift, and translational constraints, but it is usually treated separately from generalized Chargaff symmetry, the tendency for words and their reverse complements to occur at similar frequencies in long DNA sequences. Here we ask whether codon usage contains a measurable reverse-complement component, and whether departures from that component can be quantified. We first avoided imposing reverse-complement symmetry. Instead, we exhaustively evaluated all nontrivial factorized codon involutions . Across taxa, the reverse-complement transformation was the optimum , giving the highest median correlation between codon frequencies and transformed codon frequencies. Random and amino-acid-preserving reference models showed that the signal is not a generic property of codon profiles and is only partly explained by protein composition. Additional controls preserving amino-acid composition and matching GC3 in expectation showed that the observed reverse-complement correlation remains higher than expected from these constraints alone, and genus-level aggregation confirmed that the optimum is not driven by overrepresented genera. The symmetry breaks in a biologically ordered manner: the third, most degenerate codon position remains closest to the reverse-complement baseline, whereas the first departs most strongly and the second is intermediate and lineage dependent. Taxonomic comparisons reveal broad and fine-scale heterogeneity in codon-level symmetry preservation. Together, these results show that codon usage combines reverse-complement preservation with position-, lineage-, and function-dependent departures from the GCT-associated compositional baseline.