Modeling cycle phases using hormone trajectories in women with and without polyendocrine metabolic ovarian syndrome

The recent availability of at-home menstrual cycle tracking technology has created opportunities for personalized assessment of reproductive health, alongside improved characterization of hormone patterns in women with and without reproductive disorders such as polyendocrine metabolic ovarian syndrome (PMOS), which affects approximately 10% of reproductive-age women. In this study, we leverage self-tracked urinary hormone data to develop an autoregressive Hidden Markov model (arHMM) that maps cycle days to physiologically meaningful phases based on hormone trajectories. By modeling day-to-day hormonal dynamics rather than absolute hormone levels, and allowing variable phase durations, this approach accommodates substantial variability in menstrual cycles, thereby enabling meaningful comparisons within and between individuals.

Across more than 3800 cycles from over 1100 individuals, we find that arHMM-derived phases reproduce expected hormonal patterns within follicular, periovulatory, and luteal phases, and that phase-based timing for hormone testing outperforms conventional cycle day–based testing in capturing the luteinizing hormone surge and post-ovulatory progesterone rise, highlighting limitations of fixed-day clinical protocols. We identify phase-specific differences between healthy controls and individuals with self-reported PMOS, including lower luteinizing hormone in the periovulatory phase, and reduced luteal-phase progesterone levels in PMOS. Furthermore, features derived from arHMM phase assignments enable classification of PMOS status with ∼78% accuracy, demonstrating the potential of this approach for non-invasive PMOS screening.