Patterned photocatalytic proximity labelling for spatial interactomics
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Mapping protein–protein interactions and proximity within their native spatial context is essential for elucidating molecular mechanisms underlying biological processes; however, methods for spatial interactomics with subanatomical resolution are limited. Here, we introduce digital micromirror device (DMD)–PhoxID, a method that combines photocatalytic proximity labelling with patterned irradiation using a DMD to map the proximal molecular environment of proteins of interest within defined subregions of fixed brain tissue.
